» Sign in / Register

Fluorescent Nucleotides for DNA Labeling


Nucleotide Selector for DNA/RNA Labeling
Select a suitable nucleotide substrate for enzymatic DNA/RNA Labeling.

 

Table 1: Enzymatic incorporation of fluorescent Nucleotides.

PCR: Polymerase chain reaction with Taq Polymerase; MDA-based WGA: Multiple displacement amplification (MDA)-based whole genome amplification (WGA) with Phi29 DNA Polymerase; NT: Nick Translation with DNAse I / DNA Polymerase I;
Primer Extension: Primer Extension with Klenow 3'-5'exo-; 3'-End Labeling: Incorporation with Terminal deoxynucleotidyl Transferase (TdT); RT: Reverse Transcription with Moloney Murine Leukemia Virus Reverse Transcriptase (MMLV RT)


DNA Labeling cDNA
Labeling
Emission
color
NucleotideCat. No.PCR MDA-based WGA NTPrimer Extension3' End LabelingRT
dUTP
blueAMCA-6-dUTPNU-803-AMCA2n/a2n/an/an/a
DEAC-dUTPNU-803-DEAC-n/a1n/an/an/a
dUTP-ATTO-425NU-803-4251n/a1,211n/a
dUTP-XX-ATTO-425NU-803-XX-4251n/a1n/an/an/a
greendUTP-XX-AF488NU-803-XX-AF4881n/a1n/an/an/a
dUTP-XX-ATTO-488NU-803-XX-4881,2n/a1,21,21,2n/a
Fluorescein-12-dUTPNU-803-FAMX1,2n/a1,21,21,2n/a
Rhodamine-12-dUTPNU-803-RHOX-n/a1,211,2n/a
yellow-
green
dUTP-XX-ATTO-532NU-803-5321n/a111n/a
yellowdUTP-Cy3NU-803-Cy31,2n/a1,2112
dUTP-XX-Cy3NU-803-XX-CY31111n/a1
dUTP-XX-AF555NU-803-XX-AF5551n/a1n/an/an/a
dUTP-ATTO-550NU-803-550-n/a111n/a
orangedUTP-Texas RedNU-803-TXR1,2n/a1,22n/an/a
dUTP-XX-Texas RedNU-803-XX-TXR1,2n/a1,22n/an/a
dUTP-XX-AF594NU-803-XX-AF5941,2n/a1,2n/an/an/a
dUTP-XX-594NU-803-XX-594
reddUTP-Cy5NU-803-CY51,2n/a1,21n/a2
dUTP-XX-Cy5NU-803-XX-CY51111n/a1
dUTP-ATTO-647NNU-803-647N-n/a1,211n/a
dUTP-XX-643NU-803-XX-643
dUTP-XX-AF647NU-803-XX-AF6471n/a1n/an/an/a
dUTP-ATTO-655NU-803-6551n/a111n/a
dCTP
greenFluorescein-12-dCTPNU-809-FAMX1n/a1n/an/an/a
Rhodamine-12-dCTPNU-809-RHOX-n/a1n/an/an/a
yellowdCTP-Cy3NU-809-Cy31n/a1n/an/an/a
dCTP-ATTO-550NU-809-550-n/a1n/an/an/a
orangedCTP-Texas RedNU-809-TXR1n/a1n/an/an/a
reddCTP-Cy5NU-809-CY51n/a1n/an/an/a
dCTP-ATTO-647NNU-809-647N-n/a1n/an/an/a

1: in-house tested
2: external reference (see datasheet)
n/a: no data available
- : not applicable

 

Products & Ordering