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Fluorescent Nucleotides for DNA Labeling


Nucleotide Selector for DNA/RNA Labeling
Select a suitable nucleotide substrate for enzymatic DNA/RNA Labeling.

 

Table 1: Enzymatic incorporation of fluorescent Nucleotides.

PCR: Polymerase chain reaction with Taq Polymerase; MDA-based WGA: Multiple displacement amplification (MDA)-based whole genome amplification (WGA) with Phi29 DNA Polymerase; NT: Nick Translation with DNAse I / DNA Polymerase I;
Primer Extension: Primer Extension with Klenow 3'-5'exo-; 3'-End Labeling: Incorporation with Terminal deoxynucleotidyl Transferase (TdT); RT: Reverse Transcription with Moloney Murine Leukemia Virus Reverse Transcriptase (MMLV RT)


DNA Labeling cDNA
Labeling
Emission
color
NucleotideCat. No.PCR NTPrimer Extension3' End LabelingRT
dUTP
blueAMCA-6-dUTPNU-803-AMCA22n/an/an/a
DEAC-dUTPNU-803-DEAC-1n/an/an/a
dUTP-ATTO-425NU-803-42511,211n/a
dUTP-XX-ATTO-425NU-803-XX-42511n/an/an/a
greendUTP-XX-AF488NU-803-XX-AF48811n/an/an/a
dUTP-XX-ATTO-488NU-803-XX-4881,21,21,21,2n/a
Fluorescein-12-dUTPNU-803-FAMX1,21,21,21,2n/a
Rhodamine-12-dUTPNU-803-RHOX-1,211,2n/a
yellow-
green
dUTP-XX-ATTO-532NU-803-5321111n/a
yellowdUTP-Cy3NU-803-Cy31,21,2112
dUTP-XX-Cy3NU-803-XX-CY3111n/a1
dUTP-XX-AF555NU-803-XX-AF55511n/an/an/a
dUTP-ATTO-550NU-803-550-111n/a
orangedUTP-Texas RedNU-803-TXR1,21,22n/an/a
dUTP-XX-Texas RedNU-803-XX-TXR1,21,22n/an/a
dUTP-XX-AF594NU-803-XX-AF5941,21,21n/an/a
dUTP-XX-594NU-803-XX-594111
reddUTP-Cy5NU-803-CY51,21,21n/a2
dUTP-XX-Cy5NU-803-XX-CY5111n/a1
dUTP-ATTO-647NNU-803-647N-1,211n/a
dUTP-ATTO-643NU-803-643111
dUTP-XX-ATTO-643NU-803-XX-643111
dUTP-XX-AF647NU-803-XX-AF64711n/an/an/a
dUTP-ATTO-655NU-803-655-111n/a
dCTP
greenFluorescein-12-dCTPNU-809-FAMX11n/an/an/a
Rhodamine-12-dCTPNU-809-RHOX-1n/an/an/a
yellowdCTP-Cy3NU-809-Cy311n/an/an/a
dCTP-ATTO-550NU-809-550-1n/an/an/a
orangedCTP-Texas RedNU-809-TXR11n/an/an/a
reddCTP-Cy5NU-809-CY511n/an/an/a
dCTP-ATTO-647NNU-809-647N-1n/an/an/a

1: in-house tested
2: external reference (see datasheet)
n/a: no data available
- : not applicable

 

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