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Fluorescein-12-dUTP

Fluorescein-X-5-Aminoallyl-dUTP

5/6-Fluorescein-X-5-aminoallyl-2'-deoxyuridine-5'-triphosphate, Triethylammonium salt

Cat. No. Amount Price (EUR) Buy / Note
NU-803-FAMX-S 10 μl (1 mM) 84,79 Add to Basket/Quote Add to Notepad
NU-803-FAMX-L 5 x 10 μl (1 mM) 240,00 Add to Basket/Quote Add to Notepad
Structural formula of Fluorescein-12-dUTP (Fluorescein-X-5-Aminoallyl-dUTP, 5/6-Fluorescein-X-5-aminoallyl-2'-deoxyuridine-5'-triphosphate, Triethylammonium salt)
Structural formula of Fluorescein-12-dUTP
Excitation and Emission spectrum of 5/6-FAM
Excitation and Emission spectrum of 5/6-FAM

For research use only!

Shipping: shipped on blue ice

Storage Conditions: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible. If stored as recommended, Jena Bioscience guarantees optimal performance of this product for 12 months after date of delivery.

Shelf Life: 12 months

Molecular Formula: C39H41N4O21P3 (free acid)

Molecular Weight: 994.69 g/mol (free acid)

Purity: ≥ 95 % (HPLC)

Form: yellow-orange solution in water, pH 7.5 ±0.5

Concentration: 1 mM - 1.1 mM

pH: 7.5 ±0.5

Spectroscopic Properties: λexc 492 nm; λem 517 nm; ε 83.0 L mmol-1 cm-1 (Tris-HCl pH 7.5)

Applications:
Incorporation into DNA/cDNA by
- PCR with Taq polymerase in-house data, [1]
- Nick Translation with DNAse I/ DNA Polymerase I in-house data

Description:
Fluorescein-12-dUTP is recommended for direct enzymatic labeling of DNA/cDNA e.g. by PCR and Nick Translation. It is incorporated as substitute for its natural counterpart dTTP. The resulting Dye-labeled DNA/cDNA probes are ideally suited for fluorescence hybridization applications such as FISH or microarray-based gene expression profiling.Optimal substrate properties and thus labeling efficiency is ensured by an optimized linker attached to the C5 position of uridine.
Recommended Fluorescein-12-dUTP/dTTP ratio for PCR and Nick Translation: 30-50% Fluorescein-12-dUTP/ 70-50% dTTP

Please note: Protect the Dye-labeled dUTP from exposure to light and carry out experimental procedures in low light conditions. The optimal final concentration of the Dye-labeled dUTP may very depending on the application and assay conditions. For optimal product yields and high incorporation rates an individual optimization of the Dye-labeled-dUTP/dTTP ratio is recommended.

Selected References:
[1] Wiegant et al. (2001) Probe Labeling and Fluorescence in situ Hybridization. Current Protocols in Cytometry 8:8.3.1.