Fluorophores and haptens, the latter meaning Biotin, Desthiobiotin, Digoxigenin and Dinitrophenol, are the most commonly used labels for the generation of non-radioactive DNA/cDNA probes. They are enzymatically introduced into DNA/cDNA via modified nucleotides that are incorporated as substitutes for their natural counterparts in either a one-step procedure (introduction of a fluorescent or hapten-modified nucleotide) or a two-step procedure (introduction of a reactive group carrying nucleotide and subsequent fluorescent or hapten labeling).
Nucleotide Selector for DNA/RNA Labeling
Select a suitable nucleotide substrate for enzymatic DNA/RNA Labeling.
|One Step DNA/cDNA Labeling||Two Step DNA/cDNA Labeling|
|Fluorescent Labeling||Click Chemistry-based Labeling|
|Hapten Labeling||Amine Labeling|
|Template||Method||Labeled Probe||Labeling site||Enzyme|
|DNA||PCR||DNA||random||Thermophilic polymerase e.g. Taq Polymerase|
|MDA-based WGA||Phi29 DNA Polymerase|
|Nick Translation||DNAse I / DNA Polymerase I|
|Primer Extension||Klenow Fragment 3’-> 5’ exo-, Taq Polymerase|
|3’ End Labeling||3’ OH||Terminal Deoxynucleotidyl Transferase (TdT)|
|RNA||Reverse Transcription||cDNA||random||Reverse Transcriptase e.g. MMLV|