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Probes & Epigenetics

Fluorescent PCR Labeling Kits

Labeled DNA probes e.g. for (Fluorescence) in situ hybridization((F)ISH are routinely prepared via PCR if template amounts are limited or amplification of specific DNA fragments is required.

We offer High Fidelity PCR Labeling Kits ...

… for both fluorescent (e.g Cy^®-, ATTO^TM- or Alexa Fluor^®-), Biotin and Digoxigenin labeling
… suitable for preparation of both short (>100 bp) and long DNA probes (up to 4 kbp tested) due to a High Fidelity Polymerase blend consisting of Taq polymerase and a proofreading enzyme.
… with convenient configurations for application-specific assay optimization such as single nucleotide format for optimization of labeled dUTP/dTTP ratio, positive control reaction (amplification of 500 bp fragment), testkits containing selected labeled dUTPs to find the optimal label

Preview Fluorescent Dyes and Quenchers - Properties and Structures

Fluorescent Dyes and Quenchers - Properties and Structures

Products & Ordering

HighFidelity Fluorescein PCR Labeling Kit APP-101-FAMX
Preparation of Fluorescein-labeled DNA probes by PCR

HighFidelity ATTO488 PCR Labeling Kit APP-101-488
Preparation of ATTO488-labeled DNA probes by PCR

HighFidelity AF488 PCR Labeling Kit APP-101-AF488
Preparation of AF488-labeled DNA probes by PCR

HighFidelity GREEN PCR Labeling Testkit APP-101-GREEN
Preparation of FAMX-, ATTO488- and AF488-labeled DNA probes by PCR

HighFidelity Cy3 PCR Labeling Kit APP-101-CY3
Preparation of Cy3-labeled DNA probes by PCR

HighFidelity AF555 PCR Labeling Kit APP-101-AF555
Preparation of AF555-labeled DNA probes by PCR

HighFidelity YELLOW PCR Labeling Testkit APP-101-YELLOW
Preparation of Cy3- and AF555-labeled DNA probes by PCR

HighFidelity AF594 PCR Labeling Kit APP-101-AF594
Preparation of AF594-labeled DNA probes by PCR

HighFidelity ATTO594 PCR Labeling Kit APP-101-594
Preparation of ATTO594-labeled DNA probes by PCR

HighFidelity ORANGE PCR Labeling Testkit APP-101-ORANGE
Preparation of AF594-, ATTO594- and TexasRed-labeled DNA probes by PCR

HighFidelity Cy5 PCR Labeling Kit APP-101-CY5
Preparation of Cy5-labeled DNA probes by PCR

HighFidelity AF647 PCR Labeling Kit APP-101-AF647
Preparation of AF647-labeled DNA probes by PCR

HighFidelity ATTO643 PCR Labeling Kit APP-101-643
Preparation of ATTO643-labeled DNA probes by PCR

HighFidelity RED PCR Labeling Testkit APP-101-RED
Preparation of Cy5-, AF647- and ATTO643-labeled DNA probes by PCR

HighFidelity IR680LT PCR Labeling Kit APP-101-IR680LT
Preparation of IR680LT-labeled DNA probes by PCR

HighFidelity IR750 PCR Labeling Kit APP-101-IR750
Preparation of IR750-labeled DNA probes by PCR