Double-stranded (ds)RNA formation is a hallmark of viral infections that is essential for the induction of innate immunity. dsRNA is also involved in gene silencing and produced as side product during in vitro transription-based RNA synthesis. Anti-dsRNA monoclonal antibodies are efficient tools for the detection of dsRNA in cell culture & tissues such as FFPE samples as well as in vitro transcribed (m)RNA preparations [1-7] (Tab. 1) e.g.
|Anti-dsRNA monoclonal antibody J2||Mouse, IgG2a, kappa light chain|| |
ELISA, IF, FACS, IHC, IP, Dot Blot, ChIP, affinity purification, immunoelectron microscopy
|Gold standard for dsRNA detection|
Recommended for quality control of in vitro transcribed (m)RNA
|Anti-dsRNA monoclonal antibody K1||Mouse, IgG2a, kappa light chain||Recommended for Poly I:C detection|
J2 alternative in case of cross reactions
Anti-dsRNA monoclonal antibody K2
Mouse, IgM, kappa light chain
ELISA, IHC, Dot Blot
|Isotype alternative to J2 & K1 |
Recommended for (Sandwich-) ELISA
|dsRNA 142 bp||synthetic dsRNA||Positive control for Anti-dsRNA monoclonal antibodies J2, K1 and K2||n/a|
 Schönborn et al. (1991) Monoclonal antibodies to double-stranded RNA as probes of RNA structure in crude nucleic acid extracts. Nucleic Acids Res.19: 2993.
 Lukacs (1994) Detection of virus infection in plants and differentiation between coexisting viruses by monoclonal antibodies to double-stranded RNA. J. Virol. Methods47: 255.
 Lukacs (1997) Detection of sense:antisense duplexes by structure-specific anti-RNA antibodies. In: Antisense Technology. A Practical Approach, C. Lichtenstein and W. Nellen (eds), pp. 281-295. IRL Press, Oxford
 Weber et al. (2006) Double-Stranded RNA is produced by positive-strand RNA viruses and DNA viruses but not in detectable amounts by negative-strand RNA viruses. Journal of Virology 80(10): 5059.
 Knoops et al. (2008) SARS-Coronavirus replication is supported by a reticulovesicular network of modified endoplasmic reticulum. PLOS Biology 6(9): e226.
 Richardson et al. (2010) Use of antisera directed against dsRNA to detect viral infections in formalin-fixed paraffin-embedded tissue. Journal of Clinical Virology 49: 180.
 Karikó et al. (2011) Generating the optimal mRNA for therapy: HPLC purification eliminates immune activation and improves translation of nucleoside-modified, protein-encoding mRNA. Nucleic Acids Research 39(21): e142.