Double-stranded (ds)RNA formation is a hallmark of viral infections that is essential for the induction of innate immunity. dsRNA is also involved in gene silencing and produced as side product during in vitro transription-based RNA synthesis. Anti-dsRNA monoclonal antibodies are efficient tools for the detection of dsRNA in cell culture & tissues such as FFPE samples as well as in vitro transcribed (m)RNA preparations [1-7] (Tab. 1) e.g.
| Product | Description | Application | Selection guide |
|---|---|---|---|
| Anti-dsRNA monoclonal antibody J2 | Mouse, IgG2a, kappa light chain | ELISA, IF, FACS, IHC, IP, Dot Blot, ChIP, affinity purification, immunoelectron microscopy | Gold standard for dsRNA detection Recommended for quality control of in vitro transcribed (m)RNA |
| Anti-dsRNA monoclonal antibody K1 | Mouse, IgG2a, kappa light chain | Recommended for Poly I:C detection J2 alternative in case of cross reactions | |
| Anti-dsRNA monoclonal antibody K2 | Mouse, IgM, kappa light chain | ELISA, IHC, Dot Blot | Isotype alternative to J2 & K1 Recommended for (Sandwich-) ELISA |
| dsRNA 142 bp | synthetic dsRNA | Positive control for Anti-dsRNA monoclonal antibodies J2, K1 and K2 | n/a |
[1] Schönborn et al. (1991) Monoclonal antibodies to double-stranded RNA as probes of RNA structure in crude nucleic acid extracts. Nucleic Acids Res.19: 2993.
[2] Lukacs (1994) Detection of virus infection in plants and differentiation between coexisting viruses by monoclonal antibodies to double-stranded RNA. J. Virol. Methods47: 255.
[3] Lukacs (1997) Detection of sense:antisense duplexes by structure-specific anti-RNA antibodies. In: Antisense Technology. A Practical Approach, C. Lichtenstein and W. Nellen (eds), pp. 281-295. IRL Press, Oxford
[4] Weber et al. (2006) Double-Stranded RNA is produced by positive-strand RNA viruses and DNA viruses but not in detectable amounts by negative-strand RNA viruses. Journal of Virology 80(10): 5059.
[5] Knoops et al. (2008) SARS-Coronavirus replication is supported by a reticulovesicular network of modified endoplasmic reticulum. PLOS Biology 6(9): e226.
[6] Richardson et al. (2010) Use of antisera directed against dsRNA to detect viral infections in formalin-fixed paraffin-embedded tissue. Journal of Clinical Virology 49: 180.
[7] Karikó et al. (2011) Generating the optimal mRNA for therapy: HPLC purification eliminates immune activation and improves translation of nucleoside-modified, protein-encoding mRNA. Nucleic Acids Research 39(21): e142.
