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dsRNA detection

Double-stranded (ds)RNA formation is a hallmark of viral infections that is essential for the induction of innate immunity. dsRNA is also involved in gene silencing and produced as side product during in vitro transription-based RNA synthesis. Anti-dsRNA monoclonal antibodies are efficient tools for the detection of dsRNA in cell culture & tissues such as FFPE samples as well as in vitro transcribed (m)RNA preparations [1-7] (Tab. 1) e.g.

  • for characterization & detection of viruses with dsRNA genomes or intermediates (including SARS, Hepatitis C, Dengue or West Nile Virus).
  • as diagnostic tool for determination whether an unknown pathogen is of viral or bacterial origin.
  • for quality control of in vitro transcribed (m)RNA preparations.

Table 1: Overview on available dsRNA detection products

SCICONS mouse dsRNA ELISA kits (J2 based) and (K1 based) are available upon request. Just contact us!
ProductDescriptionSelection guide
Anti-dsRNA monoclonal antibody J2Mouse, IgG2a, kappa light chainGold standard for dsRNA detection
Recommended for quality control of in vitro transcribed (m)RNA
Anti-dsRNA monoclonal antibody J5Mouse, IgG2b, kappa light chainIsotope alternative to J2 with similar affinity and specificity.
Recommended for simultaneous detection of dsRNA with other markers in immunofluorescence microscopy
Anti-dsRNA monoclonal antibody K1Mouse, IgG2a, kappa light chainRecommended for Poly I:C detection
J2 alternative in case of cross reactions
Anti-dsRNA Comparison Set Samples of sizes of J2, J5 and K1 antibodiesRecommended for comparative tests of J2, J5 and K1 antibodies
Anti-dsRNA monoclonal antibody K2Mouse, IgM, kappa light chainIsotype alternative to J2 & K1
Recommended for (Sandwich-) ELISA
dsRNA 142 bpsynthetic dsRNAPositive control for Anti-dsRNA monoclonal antibodies J2, K1 and K2

Selected References

[1] Schönborn et al. (1991) Monoclonal antibodies to double-stranded RNA as probes of RNA structure in crude nucleic acid extracts. Nucleic Acids Res.19: 2993.
[2] Lukacs (1994) Detection of virus infection in plants and differentiation between coexisting viruses by monoclonal antibodies to double-stranded RNA. J. Virol. Methods47: 255.
[3] Lukacs (1997) Detection of sense:antisense duplexes by structure-specific anti-RNA antibodies. In: Antisense Technology. A Practical Approach, C. Lichtenstein and W. Nellen (eds), pp. 281-295. IRL Press, Oxford
[4] Weber et al. (2006) Double-Stranded RNA is produced by positive-strand RNA viruses and DNA viruses but not in detectable amounts by negative-strand RNA viruses. Journal of Virology 80(10): 5059.
[5] Knoops et al. (2008) SARS-Coronavirus replication is supported by a reticulovesicular network of modified endoplasmic reticulum. PLOS Biology 6(9): e226.
[6] Richardson et al. (2010) Use of antisera directed against dsRNA to detect viral infections in formalin-fixed paraffin-embedded tissue. Journal of Clinical Virology 49: 180.
[7] Karikó et al. (2011) Generating the optimal mRNA for therapy: HPLC purification eliminates immune activation and improves translation of nucleoside-modified, protein-encoding mRNA. Nucleic Acids Research 39(21): e142.