Ligase-free Cloning

Our Ligase-Free Cloning system enables fast and efficient generation of transformation-ready plasmids in just two simple steps.

Based on high-fidelity Pfu polymerase technology, the method allows seamless cloning without the need for time-consuming ligation reactions.

1) Generation of a PCR product with ends homologous to the target vector.
2) Annealing and fusion of insert and vector in a circularization reaction.

The streamlined workflow reduces hands-on time while providing reliable and efficient cloning performance for modern molecular biology applications.

No time consuming ligation step is necessary!

Contact MolBio Get in contact with us for more information!