Our Ligase-Free Cloning system enables fast and efficient generation of transformation-ready plasmids in just two simple steps.
Based on high-fidelity Pfu polymerase technology, the method allows seamless cloning without the need for time-consuming ligation reactions.
1) Generation of a PCR product with ends homologous to the target vector.
2) Annealing and fusion of insert and vector in a circularization reaction.
The streamlined workflow reduces hands-on time while providing reliable and efficient cloning performance for modern molecular biology applications.
No time consuming ligation step is necessary!
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