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Aminoallyl-dUTP-XX-ATTO-532

5-(3-Aminoallyl)-2'-deoxyuridine-5'-triphosphate, labeled with ATTO 532, Triethylammonium salt

Cat. No. Amount Price (EUR) Buy / Note
NU-803-XX-532-S 10 μl (1 mM) 102,20 Add to Basket/Quote Add to Notepad
NU-803-XX-532-L 5 x 10 μl (1 mM) 395,34 Add to Basket/Quote Add to Notepad
Structural formula of Aminoallyl-dUTP-XX-ATTO-532 (5-(3-Aminoallyl)-2'-deoxyuridine-5'-triphosphate, labeled with ATTO 532, Triethylammonium salt)
Structural formula of Aminoallyl-dUTP-XX-ATTO-532
excitation and emission spectrum of ATTO 532
excitation and emission spectrum of ATTO 532

For research use only!

Shipping: shipped on blue ice

Storage Conditions: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible.

Shelf Life: 12 months after date of delivery

Molecular Formula: C53H71N8O25P3S2 (free acid)

Molecular Weight: 1377.22 g/mol (free acid)

Exact Mass: 1376.32 g/mol (free acid)

Purity: ≥ 95 % (HPLC)

Form: sterile orange solution in 10 mM Tris-HCl

Concentration: 1.0 mM - 1.1 mM

pH: 7.5 ±0.5

Spectroscopic Properties: λexc 532 nm, λem 552 nm, ε 115.0 L mmol-1 cm-1 (Tris-HCl pH 7.5)

Applications:
Incorporation into DNA/cDNA by
- PCR with Taq polymerase in-house data
- Nick Translation with DNAse I/ DNA Polymerase I in-house data

Description:
Aminoallyl-dUTP-XX-ATTO532 is recommended for direct enzymatic labeling of DNA/cDNA e.g. by PCR and Nick Translation. It is incorporated as substitute for its natural counterpart dTTP. The resulting Dye-labeled DNA/cDNA probes are ideally suited for fluorescence hybridization applications such as FISH or microarray-based gene expression profiling.Optimal substrate properties and thus labeling efficiency is ensured by an optimized linker attached to the C5 position of uridine.

Recommended Aminoallyl-dUTP-XX-ATTO532/dTTP ratio for PCR and Nick Translation: 30-50% Aminoallyl-dUTP-XX-ATTO532/ 50% dTTP

Please note: Protect the Dye-labeled dUTP from exposure to light and carry out experimental procedures in low light conditions. The optimal final concentration of the Dye-labeled dUTP may very depending on the application and assay conditions. For optimal produdct yields and high incorporation rates an individual optimization of the Dye-labeled-dUTP/dTTP ratio is recommended.