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Aminoallyl-dUTP-XX - AF488

also known as Alexa Fluor 488®-dUTP

5-(3-Aminoallyl)-2'-deoxyuridine-5'-triphosphate, labeled with AF488, Triethylammonium salt

Cat. No. Amount Price (EUR) Buy / Note
NU-803-XX-AF488-S 10 μl (1 mM) 98,50 Add to Basket/Quote Add to Notepad
NU-803-XX-AF488-L 5 x 10 μl (1 mM) 389,50 Add to Basket/Quote Add to Notepad
Structural formula of Aminoallyl-dUTP-XX - AF488 (also known as Alexa Fluor 488®-dUTP, 5-(3-Aminoallyl)-2'-deoxyuridine-5'-triphosphate, labeled with AF488, Triethylammonium salt)
Structural formula of Aminoallyl-dUTP-XX - AF488

For research use only!

Shipping: shipped on blue ice

Storage Conditions: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible. If stored as recommended, Jena Bioscience guarantees optimal performance of this product for 12 months after date of delivery.

Shelf Life: 12 months

Molecular Formula: C45H54N7O26P3S2 (free acid)

Molecular Weight: 1265.99 g/mol (free acid)

Purity: ≥ 95 % (HPLC)

Form: sterile yellow solution in 10 mM Tris-HCl, pH 7.5 ±0.5

Concentration: 1 mM - 1.1 mM

pH: 7.5 ±0.5

Spectroscopic Properties: λexc 494 nm; λem 515 nm; ε 73.0 L mmol-1 cm-1 (Tris-HCl pH 7.5)

Applications:
Incorporation into DNA/cDNA by
- PCR with Taq polymerase in-house data
- Nick Translation with DNAse I/ DNA Polymerase I in-house data

Description:
Aminoallyl-dUTP-XX-AF488 is recommended for direct enzymatic labeling of DNA/cDNA e.g. by PCR and Nick Translation. It is incorporated as substitute for its natural counterpart dTTP. The resulting Dye-labeled DNA/cDNA probes are ideally suited for fluorescence hybridization applications such as FISH or microarray-based gene expression profiling.Optimal substrate properties and thus labeling efficiency is ensured by an optimized linker attached to the C5 position of uridine. AF488 (also known as Alexa Fluor 488®) is a hydrophilic dye with excellent photostability compared to fluorescein.

Recommended Aminoallyl-dUTP-XX-AF488/dTTP ratio for PCR and Nick Translation: 30-50% Aminoallyl-dUTP-XX-AF488/ 70-50% dTTP

Please note: Protect the Dye-labeled dUTP from exposure to light and carry out experimental procedures in low light conditions. The optimal final concentration of the Dye-labeled dUTP may very depending on the application and assay conditions. For optimal product yields and high incorporation rates an individual optimization of the Dye-labeled-dUTP/dTTP ratio is recommended.