Rho-green-X-dUTP, RhG-dUTP, R110-dUTP
5/6-Rhodamine-X-(5-aminoallyl)-2'-deoxyuridine-5'-triphosphate, Triethylammonium salt
For general laboratory use.
Shipping: shipped on gel packs
Storage Conditions: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible.
Shelf Life: 12 months after date of delivery
Molecular Formula: C39H43N6O19P3 (free acid)
Molecular Weight: 992.71 g/mol (free acid)
Exact Mass: 992.18 g/mol (free acid)
Purity: ≥ 95 % (HPLC)
Form: solution in water
Concentration: 1.0 mM - 1.1 mM
pH: 7.5 ±0.5
Spectroscopic Properties: λexc 505 nm, λem 530 nm, ε 85.0 L mmol-1 cm-1 (Tris-HCl pH 7.5), CF280 nm 0.27, CF260 nm 0.39
Incorporation into DNA/cDNA by
- Nick Translation with DNAse I/ DNA Polymerase I in-house data
Rhodamine-12-dUTP is recommended for direct enzymatic labeling of DNA/cDNA by Nick Translation. It is incorporated as substitute for its natural counterpart dTTP. The resulting Dye-labeled DNA/cDNA probes are ideally suited for fluorescence hybridization applications such as FISH or microarray-based gene expression profiling. Optimal substrate properties and thus labeling efficiency is ensured by an optimized linker attached to the C5 position of uridine.
Recommended Rhodamine-12-dUTP/dTTP ratio for Nick Translation: 30-50% Rhodamine-12-dUTP/ 70-50% dTTP
Please note: Protect the Dye-labeled dUTP from exposure to light and carry out experimental procedures in low light conditions. The optimal final concentration of the Dye-labeled dUTP may very depending on the application and assay conditions. For optimal product yields and high incorporation rates an individual optimization of the Dye-labeled-dUTP/dTTP ratio is recommended.
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