» Sign in / Register

Aminoallyl-dUTP-XX-Cy5

5-(3-Aminoallyl)-2'-deoxyuridine-5'-triphosphate, labeled with Cy5, Triethylammonium salt

Cat. No. Amount Price (EUR) Buy / Note
NU-803-XX-CY5-S 10 μl (1 mM) 86,06 Add to Basket/Quote Add to Notepad
NU-803-XX-CY5-L 5 x 10 μl (1 mM) 332,92 Add to Basket/Quote Add to Notepad
Structural formula of Aminoallyl-dUTP-XX-Cy5 (5-(3-Aminoallyl)-2'-deoxyuridine-5'-triphosphate, labeled with Cy5, Triethylammonium salt)
Structural formula of Aminoallyl-dUTP-XX-Cy5
Excitation and Emission spectrum of Cy5
Excitation and Emission spectrum of Cy5

For research use only!

Shipping: shipped on blue ice

Storage Conditions: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible.

Shelf Life: 12 months after date of delivery

Molecular Formula: C57H80N7O23P3S2 (free acid)

Molecular Weight: 1388.33 g/mol (free acid)

Purity: ≥ 95 % (HPLC)

Form: sterile blue solution in 10 mM Tris-HCl

Concentration: 1.0 mM - 1.1 mM

pH: 7.5 ±0.5

Spectroscopic Properties: λexc 649 nm, λem 670 nm, ε 250.0 L mmol-1 cm-1 (Tris-HCl pH 7.5)

Applications:
Incorporation into DNA/cDNA by
- PCR with Taq polymerase in-house data
- Nick Translation with DNAse I/ DNA Polymerase I in-house data

Description:
Aminoallyl-dUTP-XX-Cy5 is recommended for direct enzymatic labeling of DNA/cDNA e.g. by PCR and Nick Translation. It is incorporated as substitute for its natural counterpart dTTP. The resulting Dye-labeled DNA/cDNA probes are ideally suited for fluorescence hybridization applications such as FISH or microarray-based gene expression profiling. Optimal substrate properties and thus labeling efficiency is ensured by an optimized linker attached to the C5 position of uridine. Higher incorporation and thus labeling rates can be achieved compared to Aminoally-dUTP-Cy5 (NU-803-Cy5).

Recommended Aminoallyl-dUTP-XX-Cy5/dTTP ratio for PCR and Nick Translation: 20-40% Aminoallyl-dUTP-XX-Cy5/ 80-60% dTTP

Please note: Protect the Dye-labeled dUTP from exposure to light and carry out experimental procedures in low light conditions. The optimal final concentration of the Dye-labeled dUTP may very depending on the application and assay conditions. For optimal produdct yields and high incorporation rates an individual optimization of the Dye-labeled-dUTP/dTTP ratio is recommended.