» Sign in / Register

Protein Labeling

The attachment of a reporter group (label) is often required for the detection and biochemical/cellular characterization of proteins and its binding partners.

Different labeling techniques & types of label such as fluorophores, biotin and luminescent dyes are available for non-radioactive protein labeling, both random and site-directed (N- or C-terminal).

Choose the appropriate labeling strategy (labeling technique & type of label) that fits your final application based on your initial protein source (Tab. 1).

Table 1: Protein Labeling Selection Matrix



Type of Label



FluorophoreBiotin
Desthiobiotin
CLICK functionality
Protein SourcePurified protein Amine
Labeling
Amine
Labeling

Amine
Labeling
randomLabeling Position
Thiol
Labeling

Thiol
Labeling
Proliferating cells
(Nascent proteins)



Amino acid-based
Metabolic Labeling



Puromycin-based
Metabolic Labeling
site directed

ATP dependent
Lysine-based Labeling
ATP dependent
Lysine-based Labeling

Protein encoding DNA sequence
(Cloning & cell-free expression required)
dC-Puromycin-based Co-translational Labeling dC-Puromycin-based Co-translational Labeling