The attachment of a reporter group (label) is often required for the detection and biochemical/cellular characterization of proteins and its binding partners.
Different labeling techniques & types of label such as fluorophores, biotin and luminescent dyes are available for non-radioactive protein labeling, both random and site-directed (N- or C-terminal).
Choose the appropriate labeling strategy (labeling technique & type of label) that fits your final application based on your initial protein source (Tab. 1).
|Type of Label|
|Protein Source||Purified protein|| Amine|
|Protein encoding DNA sequence |
(Cloning & cell-free expression required)
|dC-Puromycin-based Co-translational Labeling||dC-Puromycin-based Co-translational Labeling|