Aminoallyl-dUTP-ATTO-425

Excitation and Emission spectrum of ATTO 425

For general laboratory use.

Shipping: shipped on gel packs

Storage Conditions: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible.

Shelf Life: 12 months after date of delivery

Molecular Formula: C₃₄H₄₅N₄O₁₉P₃ (free acid)

Molecular Weight: 906.66 g/mol (free acid)

Exact Mass: 906.16 g/mol (free acid)

Purity: ≥ 95 % (HPLC)

Form: solution in water

Color: green-yellow

Concentration: 1.0 mM - 1.1 mM

pH: 7.5 ±0.5

Spectroscopic Properties: λ_exc 439 nm, λ_em 485 nm, ε 45.0 L mmol^-1 cm^-1 (Tris-HCl pH 7.5)

Applications:
Incorporation into DNA/cDNA by
- PCR with Taq polymerase ^{in-house data}
- Nick Translation with DNAse I/ DNA Polymerase I ^{in-house data}

Description:
Aminoallyl-dUTP-ATTO425 is recommended for direct enzymatic labeling of DNA/cDNA e.g. by PCR and Nick Translation. It is incorporated as substitute for its natural counterpart dTTP. The resulting Dye-labeled DNA/cDNA probes are ideally suited for fluorescence hybridization applications such as FISH or microarray-based gene expression profiling.Optimal substrate properties and thus labeling efficiency is ensured by an optimized linker attached to the C5 position of uridine.

Recommended Aminoallyl-dUTP-ATTO425/dTTP ratio for PCR and Nick Translation: 20-30% Aminoallyl-dUTP-ATTO425/ 80-70% dTTP (PCR), 30-50% Aminoallyl-dUTP-ATTO425/ 70-50% dTTP (Nick Translation)

Please note: Protect the Dye-labeled dUTP from exposure to light and carry out experimental procedures in low light conditions. The optimal final concentration of the Dye-labeled dUTP may very depending on the application and assay conditions. For optimal product yields and high incorporation rates an individual optimization of the Dye-labeled-dUTP/dTTP ratio is recommended.

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Selected References:
Basinko et al. (2012) Clinical and molecular cytogenetic studies in ring chromosome 5: report of a child with congenital abnormalities.Eur. J. Med. Genet. 55 (2):112.