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Aminoallyl-dUTP-ATTO-425

5-(3-Aminoallyl)-2'-deoxyuridine-5'-triphosphate, labeled with ATTO 425, Triethylammonium salt

Cat. No. Amount Price (EUR) Buy / Note
NU-803-425-S 10 μl (1 mM) 99,98 Add to Basket/Quote Add to Notepad
NU-803-425-L 5 x 10 μl (1 mM) 395,34 Add to Basket/Quote Add to Notepad
Structural formula of Aminoallyl-dUTP-ATTO-425 (5-(3-Aminoallyl)-2'-deoxyuridine-5'-triphosphate, labeled with ATTO 425, Triethylammonium salt)
Structural formula of Aminoallyl-dUTP-ATTO-425
Excitation and Emission spectrum of ATTO 425
Excitation and Emission spectrum of ATTO 425

For research use only!

Shipping: shipped on blue ice

Storage Conditions: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible.

Shelf Life: 12 months after date of delivery

Molecular Formula: C34H45N4O19P3 (free acid)

Molecular Weight: 906.66 g/mol (free acid)

Exact Mass: 906.16 g/mol (free acid)

Purity: ≥ 95 % (HPLC)

Form: green-yellow solution in water

Concentration: 1.0 mM - 1.1 mM

pH: 7.5 ±0.5

Spectroscopic Properties: λexc 439 nm, λem 485 nm, ε 45.0 L mmol-1 cm-1 (Tris-HCl pH 7.5)

Applications:
Incorporation into DNA/cDNA by
- PCR with Taq polymerase in-house data
- Nick Translation with DNAse I/ DNA Polymerase I in-house data

Description:
Aminoallyl-dUTP-ATTO425 is recommended for direct enzymatic labeling of DNA/cDNA e.g. by PCR and Nick Translation. It is incorporated as substitute for its natural counterpart dTTP. The resulting Dye-labeled DNA/cDNA probes are ideally suited for fluorescence hybridization applications such as FISH or microarray-based gene expression profiling.Optimal substrate properties and thus labeling efficiency is ensured by an optimized linker attached to the C5 position of uridine.

Recommended Aminoallyl-dUTP-ATTO425/dTTP ratio for PCR and Nick Translation: 20-30% Aminoallyl-dUTP-ATTO425/ 80-70% dTTP (PCR), 30-50% Aminoallyl-dUTP-ATTO425/ 70-50% dTTP (Nick Translation)

Please note: Protect the Dye-labeled dUTP from exposure to light and carry out experimental procedures in low light conditions. The optimal final concentration of the Dye-labeled dUTP may very depending on the application and assay conditions. For optimal product yields and high incorporation rates an individual optimization of the Dye-labeled-dUTP/dTTP ratio is recommended.

Product Citations:
Please click the black arrow on the right to expand the citation list. Click publication title for the full text.

Selected References:
Basinko et al. (2012) Clinical and molecular cytogenetic studies in ring chromosome 5: report of a child with congenital abnormalities.Eur. J. Med. Genet. 55 (2):112.