The term GTPase, GTP-binding protein - or simply G-protein - is used for enzymes that are involved in cellular signal transduction by utilizing the nucleotide GTP. They consist of 2 major subgroups called *Heterotrimeric G-proteins* and *Small GTPases*, and their activities are modulated by GEFs and GAPs in a cycling fashion (see figure below).
Traditionally, measuring the activation state of a small GTPase in vivo (i.e. the GTPase-GDP/GTP levels) required tedious 32P-labelling of cells, enzyme isolation and HPLC of bound GDP and GTP. In contrast, non-radioactive pull down assays allow very easy determination, especially since GTPase Activation Kits are availability.
In addition, we offer specific monoclonal and polyclonal GDP/GTP Binding Antibodies of the Ras- and Rho/Rac/Cdc42 family. All antibodies are recommended for Western Blot, Immunohistochemistry, Immunofluoerescence, Immunoprecipitation (IP) and ELISA.
Simplified Illustration of GTP Signal Transduction - find out more by clicking on the icons