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Aminoallyl-dUTP-ATTO-647N

5-(3-Aminoallyl)-2'-deoxyuridine-5'-triphosphate, labeled with ATTO 647N, Triethylammonium salt

Cat. No. Amount Price (EUR) Buy / Note
NU-803-647N-S 10 μl (1 mM) 99,98 Add to Basket/Quote Add to Notepad
NU-803-647N-L 5 x 10 μl (1 mM) 395,34 Add to Basket/Quote Add to Notepad
Structural formula of Aminoallyl-dUTP-ATTO-647N (5-(3-Aminoallyl)-2'-deoxyuridine-5'-triphosphate, labeled with ATTO 647N, Triethylammonium salt)
Structural formula of Aminoallyl-dUTP-ATTO-647N
excitation and emission spectrum of ATTO 647N
excitation and emission spectrum of ATTO 647N

For research use only!

Shipping: shipped on blue ice

Storage Conditions: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible.

Shelf Life: 12 months after date of delivery

Molecular Formula: C58H78N9O15P3 (free acid)

Molecular Weight: 1151.08 g/mol (free acid)

Exact Mass: 1150.40 g/mol (free acid)

Purity: ≥ 95 % (HPLC)

Form: sterile blue solution in 10 mM Tris-HCl

Concentration: 1.0 mM - 1.1 mM

pH: 7.5 ±0.5

Spectroscopic Properties: λexc 646 nm, λem 664 nm, ε 150.0 L mmol-1 cm-1 (Tris-HCl pH 7.5)

Applications:
Incorporation into DNA/cDNA by
- Nick Translation with DNAse I/ DNA Polymerase I in-house data

Description:
Aminoallyl-dUTP-ATTO647N is recommended for direct enzymatic labeling of DNA/cDNA by Nick Translation. It is incorporated as substitute for its natural counterpart dTTP. The resulting Dye-labeled DNA/cDNA probes are ideally suited for fluorescence hybridization applications such as FISH or microarray-based gene expression profiling. Optimal substrate properties and thus labeling efficiency is ensured by an optimized linker attached to the C5 position of uridine.
Recommended Aminoallyl-dUTP-ATTO647N/dTTP ratio for Nick Translation: 30-50% Aminoallyl-dUTP-ATTO647N/ 50% dTTP

Please note: Protect the Dye-labeled dUTP from exposure to light and carry out experimental procedures in low light conditions. The optimal final concentration of the Dye-labeled dUTP may very depending on the application and assay conditions. For optimal produdct yields and high incorporation rates an individual optimization of the Dye-labeled-dUTP/dTTP ratio is recommended.

Product Citations:
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