5-(3-Aminoallyl)-2'-deoxyuridine-5'-triphosphate, labeled with AF555, Triethylammonium salt
For general laboratory use.
Shipping: shipped on gel packs
Storage Conditions: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible.
Shelf Life: 12 months after date of delivery
Molecular Formula: C24H41N5O16P3 - AF555 (free acid)
Molecular Weight: 1577.50 g/mol (free acid)
Purity: ≥ 95 % (HPLC)
Form: filtered solution (30 kDa) in 10 mM Tris-HCl
Concentration: 1.0 mM - 1.1 mM
pH: 7.5 ±0.5
Spectroscopic Properties: λexc 555 nm, λem 572 nm, ε 155.0 L mmol-1 cm-1 (Tris-HCl pH 7.5)
Incorporation into DNA/cDNA by
- PCR with Taq polymerase in-house data
- Nick Translation with DNAse I/ DNA Polymerase I in-house data
Aminoallyl-dUTP-XX-AF555 is recommended for direct enzymatic labeling of DNA/cDNA e.g. by PCR and Nick Translation. It is incorporated as substitute for its natural counterpart dTTP. The resulting Dye-labeled DNA/cDNA probes are ideally suited for fluorescence hybridization applications such as FISH or microarray-based gene expression profiling.Optimal substrate properties and thus labeling efficiency is ensured by an optimized linker attached to the C5 position of uridine. AF555 (structural analog to Alexa Fluor 555®) is a hydrophilic dye with excellent photostability compared to fluorescein.
Recommended Aminoallyl-dUTP-XX-AF555/dTTP ratio for PCR and Nick Translation: 30-50% Aminoallyl-dUTP-XX-AF555/ 70-50% dTTP
Please note: Protect the Dye-labeled dUTP from exposure to light and carry out experimental procedures in low light conditions. The optimal final concentration of the Dye-labeled dUTP may very depending on the application and assay conditions. For optimal product yields and high incorporation rates an individual optimization of the Dye-labeled-dUTP/dTTP ratio is recommended.
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