The introduction of a fluorophore, a biotin/desthiobiotin moiety or a reactive group is often required for the subsequent detection, purification or crosslinking of a biomolecule. This is routinely achieved by a standardized set of chemical reaction pairs (Tab. 1).
Choose from a number of reactive components the most suitable for your application.
|Reactive group||Reaction partner|
|NHS ester||Primary amino group (-NH2)|
|Maleimide||Thiol group (-SH)|
|Azide||terminal or strained Alkyne (e.g. DBCO)|
|DBCO (strained Alkyne)||Azide|
|Tetrazine||strained Alkene (e.g.Trans-cyclooctene)|