The introduction of a fluorophore, a biotin/desthiobiotin moiety or a reactive group is often required for the subsequent detection, purification or crosslinking of a biomolecule. This is routinely achieved by a standardized set of chemical reaction pairs (Tab. 1).
Choose from a number of reactive components the most suitable for your application.
| Reactive group | Reaction partner |
|---|---|
| NHS ester | Primary amino group (-NH2) |
| Maleimide | Thiol group (-SH) |
| Azide | terminal or strained Alkyne (e.g. DBCO) |
| terminal Alkyne | Azide |
| DBCO (strained Alkyne) | Azide |
| Tetrazine | strained Alkene (e.g.Trans-cyclooctene) |
| Trans-cyclooctene | Tetrazine |
