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The introduction of a fluorophore, a biotin/desthiobiotin moiety or a reactive group is often required for the subsequent detection, purification or crosslinking of a biomolecule. This is routinely achieved by a standardized set of chemical reaction pairs (Tab. 1).

Choose from a number of reactive components the most suitable for your application.

Table 1: Overview of chemical reaction pairs.

Reactive groupReaction partner
NHS esterPrimary amino group (-NH2)
MaleimideThiol group (-SH)
Azideterminal or strained Alkyne (e.g. DBCO)
terminal AlkyneAzide
DBCO (strained Alkyne) Azide
Tetrazinestrained Alkene (e.g.Trans-cyclooctene)
Trans-cycloocteneTetrazine