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Reverse Transcription & RT-PCR

In this section you can find everything related to classical Reverse Transcription and realtime RT-PCR. We offer you liquid RT-qPCR master mixes for Direct Amplification, for Detection with TaqMan® Probes or with Sybr®Green as well as Lyophilisates and Dual labelled Fluorescent Probes. Master mixes and Components for Endpoint reactions and cDNA synthesis are all available in the section Components & Classics.

All our master mixes contain polymerases and in house produced nucleotides of highest quality. Master mixes based on the detection with SYBR® Green (an intercalating dye) are most useful for setting up Singleplex reactions, whereas Mixes based on the usage of dual labelled probes (TaqMan® Detection) can also be used for the highly specific, simultaneous detection of multiple targets.

From crude extract to quantitative analysis – this can be reached fastest by our Direct Amplification master mixes which come along with an extraction buffer compatible to the subsequent RT-qPCR.

You are interested in freeze dried master mixes to avoid cooling chains and storage issues – check out the lyophilised version of our RT-qPCR ProbesMaster.

You need more than the standard package size or only single components of our systems? Get in contact with us to discuss customization from bulk filling to OEM production (molbio@jenabioscience.com).