The unicellular kinetoplast protozoan Leishmania tarentolae, isolated from the Moorish gecko Tarentola mauritanica, not pathogenic to mammalians (Biosafety level 1) – was turned into the protein-producing host of our eukaryotic protein expression system LEXSY:
- eukaryotic host as easy to handle as E. coli: no specific labware, no cell biology equipment required
- fully eukaryotic protein expression machinery with post-translational modifications, including glycosylation and disulfide bond formation
- shuttle vectors: cloning in E. coli, expression in LEXSY host
- constitutive or inducible, intracellular or secretory expression of target proteins
- stable expression strains for constant protein production
Using the Protozoan Leishmania tarentolae as a Protein Expression System
Talk held at HKI Colloquium in Jena, May 15, 2018
