5-(3-Aminoallyl)-2'-deoxyuridine-5'-triphosphate, labeled with Cy3, Triethylammonium salt
For research use only!
Shipping: shipped on blue ice
Storage Conditions: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible.
Shelf Life: 12 months after date of delivery
Molecular Formula: C43H56N5O21P3S2 (free acid)
Molecular Weight: 1135.97 g/mol (free acid)
Exact Mass: 1135.21 g/mol (free acid)
Purity: ≥ 95 % (HPLC)
Form: sterile pink solution in 10 mM Tris-HCl
Concentration: 1.0 mM - 1.1 mM
pH: 7.5 ±0.5
Spectroscopic Properties: λexc 550 nm, λem 570 nm, ε 150.0 L mmol-1 cm-1 (Tris-HCl pH 7.5), CF260 0.08
Incorporation into DNA/cDNA by
- Primer Extension with Klenow fragment
- PCR with Taq polymerase in-house data
- Nick Translation with DNAse I/ DNA Polymerase I in-house data
Aminoallyl-dUTP-Cy3 is recommended for direct enzymatic labeling of DNA/cDNA e.g. by PCR and Nick Translation. It is incorporated as substitute for its natural counterpart dTTP. The resulting Dye-labeled DNA/cDNA probes are ideally suited for fluorescence hybridization applications such as FISH or microarray-based gene expression profiling. Optimal substrate properties and thus labeling efficiency is ensured by an optimized linker attached to the C5 position of uridine.
Recommended Aminoallyl-dUTP-Cy3/dTTP ratio for PCR and Nick Translation: 30-50% Aminoallyl-dUTP-Cy3/ 50% dTTP
Please note: Protect the Dye-labeled dUTP from exposure to light and carry out experimental procedures in low light conditions. The optimal final concentration of the Dye-labeled dUTP may very depending on the application and assay conditions. For optimal produdct yields and high incorporation rates an individual optimization of the Dye-labeled-dUTP/dTTP ratio is recommended.
Please click the black arrow on the right to expand the citation list. Click publication title for the full text.
 Walsh et al. (2017) Measurement of incorporation kinetics of non-fluorescent native nucleotides by DNA polymerases using fluorescence microscopy. Nucleic Acids Res. 45 (21):e175.