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3'-End RNA Labeling (T4 RNA Ligase 1-based)

One-step labeling of short RNA is achieved via T4 RNA Ligase 1-mediated incorporation of labeled pCps.

AF (Alexa Fluor®)- and ATTO® dyes are hydrophilic fluorescent dyes with remarkable photostability and thus ideally suited for fluorescence imaging applications. Biotin- and Desthiobiotin labeling allows efficient RNA pull-down.