5-Ethynyl-UTP (5-EUTP)

For general laboratory use.

Shipping: shipped on gel packs

Storage Conditions: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible.

Shelf Life: 12 months after date of delivery

Molecular Formula: C₁₁H₁₅N₂O₁₅P₃ (free acid)

Molecular Weight: 508.16 g/mol (free acid)

Exact Mass: 507.97 g/mol (free acid)

Purity: ≥ 95 % (HPLC)

Form: solution in water

Color: colorless to slightly yellow

Concentration: 100 mM - 110 mM

pH: 7.5 ±0.5

Spectroscopic Properties: λ_max 288 nm, ε 12.0 L mmol^-1 cm^-1 (Tris-HCl pH 7.5)

Applications:
Incorporation into RNA by T7 RNA polymerase-mediated in vitro transcription.
The resulting alkyne-functionalized RNA can subsequently be processed via Cu(I)-catalyzed (azide-alkyne) click chemistry that offers the choice

• to introduce a Biotin group for subsequent purification tasks (via Azides of Biotin)
• to introduce fluorescent group for subsequent microscopic imaging (via Azides of fluorescent dyes)
• to crosslink the RNA to azide-functionalized biomolecules e.g.proteins

Presolski et al.^[1] and Hong et al.^[2] provide a general protocol for Cu(I)-catalyzed click chemistry reactions that may be used as a starting point for the set up and optimization of individual assays.

Related products:

• Copper (II)-Sulphate (CuSO₄), #CLK-MI004
• Tris(3-hydroxypropyltriazolylmethyl)amine (THPTA), #CLK-1010
• Sodium Ascorbate (Na-Ascorbate), #CLK-MI005

BIOZ Product Citations:

Selected References:
[1] Presolski et al. (2011) Copper-Catalyzed Azide-Alkyne Click Chemistry for Bioconjugation. Current Protocols in Chemical Biology 3:153.
[2] Hong et al. (2011) Analysis and Optimization of Copper-Catalyzed Azide-Alkyne Cycloaddition for Bioconjugation. Angew. Chem. Int. Ed. 48:9879.