Na-Ascorbate - click chemistry grade

For general laboratory use.

Shipping: shipped at ambient temperature

Storage Conditions: store at ambient temperature
store dry

Shelf Life: 12 months after date of delivery

Molecular Formula: C₆H₇NaO₆

Molecular Weight: 198.11 g/mol

CAS#: 134-03-2

Form: solid

Color: white to off-white

Solubility: water

Description:
Na-Ascorbate can be used as a reduction reagent for Cu(I)-catalyzed Alkyne-Azide click chemistry reactions (CuAAC).

It catalyzes the reduction of Cu(II) sources such as CuSO₄ thereby releasing catalytically reactive Cu(I) ions.

Ideally, solutions should be freshly prepared in ddH₂O shortly before use. Alternatively, a stock solution can be prepared, stored at -20°C and freshly by diluted shortly before use.

Please note: Do not use solutions that appear brown. Freshly prepared, fully functional Na-Ascorbate solutions are colorless and turn brown upon oxidization thereby loosing their reduction capability.

Presolski et al.^[1] and Hong et al.^[2] provide a general protocol for CuAAC reactions that may be used as a starting point for the set up and optimization of individual assays.

Related products:

• Copper (II)-Sulphate (CuSO₄), #CLK-MI004
• THPTA, #CLK-1010
• BTTAA, #CLK-067

BIOZ Product Citations:

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Selected References:
[1] Presolski et al. (2011) Copper-Catalyzed Azide-Alkyne Click Chemistry for Bioconjugation. Current Protocols in Chemical Biology 3:153.
[2] Hong et al. (2011) Analysis and Optimization of Copper-Catalyzed Azide-Alkyne Cycloaddition for Bioconjugation. Angew. Chem. Int. Ed. 48:9879.