Lamivudine triphosphate, Sodium Salt (L-Isomer)
2', 3'-Dideoxy-3'-thia-cytidine-5'-triphosphate, Sodium salt (L isomer)
For general laboratory use.
Shipping: shipped on gel packs
Storage Conditions: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible.
Shelf Life: 12 months after date of delivery
Molecular Formula: C8H14N3O12P3S (free acid)
Molecular Weight: 469.19 g/mol (free acid)
Exact Mass: 468.95 g/mol (free acid)
Purity: ≥ 95 % (HPLC)
Form: solution in water
Color: colorless to slightly yellow
Concentration: 10 mM - 11 mM
pH: 7.5 ±0.5
Spectroscopic Properties: λabs 270 nm, ε 9.5 L mmol-1 cm-1 (Tris-HCl pH 7.5)
Monitoring in mononuclear cells by ion-pair LC and MS
Influence on incorporation efficiency
Inhibition of DNA/RNA-dependent DNA-polymerase
BIOZ Product Citations:
 Coulier et al. (2008) Simultaneous determination of endogenous deoxynucleotides and phosphorylated nucleoside reverse transcriptase inhibitors in peripheral blood mononuclear cells using ion-pair liquid chromatography coupled to mass spectrometry. Proteomics Clin. Appl. 2:1557.
 Lim et al. (2001) Differential incorporation and removal of antiviral deoxynucleotides by human DNA polymerase y. J. Biol. Chem. 276 (26):23616.
 Hart et al. (1992) Effects of (-)-2-deoxy-3-thiacytidine (3TC) 5-triphosphate on human immunodeficiency virus reverse transcriptase and mammalian DNA polymerases alpha, beta and gamma. Antimicrobial Agents and Chemotherapy 36 (8):1688.
Boyer et al. (2001) YADD mutants of human immunodeficiency virus type 1 and Moloney murine leukemia virus reverse transcriptase are resistant to lamivudine triphosphate (3TCTP) in vitro. J. Virol. 75:6321.
Feng et al. (1999) Mechanistic studies comparing the incorporation of (+) and (-) isomers of 3TCTP by HIV-1 reverse transcriptase. Biochemistry 38:55.
Kewn et al. (2002) Development of enzymatic assays for quantification of intracellular lamivudine and carbovir triphosphate levels in peripheral blood mononuclear cells from human immunodeficiency virus-infected patients. Antimicrob. Agents Chemother. 46:135.