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3TCTP

Lamivudine triphosphate, Sodium Salt (L-Isomer)

2', 3'-Dideoxy-3'-thia-cytidine-5'-triphosphate, Sodium salt (L isomer)

Cat. No. Amount Price (EUR) Buy / Note
NU-1606S 5 μl (10 mM) 117,35 Add to Basket/Quote Add to Notepad
NU-1606L 5 x 5 μl (10 mM) 343,76 Add to Basket/Quote Add to Notepad
Structural formula of 3TCTP (Lamivudine triphosphate, Sodium Salt (L-Isomer), 2', 3'-Dideoxy-3'-thia-cytidine-5'-triphosphate, Sodium salt (L isomer))
Structural formula of 3TCTP

For research use only!

Shipping: shipped on blue ice

Storage Conditions: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible.

Shelf Life: 12 months after date of delivery

Molecular Formula: C8H14N3O12P3S (free acid)

Molecular Weight: 469.19 g/mol (free acid)

Exact Mass: 468.95 g/mol (free acid)

CAS#: 143188-53-8

Purity: ≥ 95 % (HPLC)

Form: clear aqueous solution

Concentration: 10 mM - 11 mM

pH: 7.5 ±0.5

Spectroscopic Properties: λabs 270 nm, ε 9.5 L mmol-1 cm-1 (Tris-HCl pH 7.5)

Applications:
Monitoring in mononuclear cells by ion-pair LC and MS[1]
Influence on incorporation efficiency[2]
Inhibition of DNA/RNA-dependent DNA-polymerase[3]

Product Citations:
Please click the black arrow on the right to expand the citation list. Click publication title for the full text.

Selected References:
[1] Coulier et al. (2008) Simultaneous determination of endogenous deoxynucleotides and phosphorylated nucleoside reverse transcriptase inhibitors in peripheral blood mononuclear cells using ion-pair liquid chromatography coupled to mass spectrometry. Proteomics Clin. Appl. 2:1557.
[2] Lim et al. (2001) Differential incorporation and removal of antiviral deoxynucleotides by human DNA polymerase y. J. Biol. Chem. 276 (26):23616.
[3] Hart et al. (1992) Effects of (-)-2-deoxy-3-thiacytidine (3TC) 5-triphosphate on human immunodeficiency virus reverse transcriptase and mammalian DNA polymerases alpha, beta and gamma. Antimicrobial Agents and Chemotherapy 36 (8):1688.
Boyer et al. (2001) YADD mutants of human immunodeficiency virus type 1 and Moloney murine leukemia virus reverse transcriptase are resistant to lamivudine triphosphate (3TCTP) in vitro. J. Virol. 75:6321.
Feng et al. (1999) Mechanistic studies comparing the incorporation of (+) and (-) isomers of 3TCTP by HIV-1 reverse transcriptase. Biochemistry 38:55.
Kewn et al. (2002) Development of enzymatic assays for quantification of intracellular lamivudine and carbovir triphosphate levels in peripheral blood mononuclear cells from human immunodeficiency virus-infected patients. Antimicrob. Agents Chemother. 46:135.