3TCTP
For research use only.
Please centrifuge briefly before opening (volume ≤2 ml).
Shipping: shipped on gel packs
Storage Conditions: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible.
Shelf Life: 12 months after date of delivery
Molecular Formula: C8H14N3O12P3S (free acid)
Molecular Weight: 469.19 g/mol (free acid)
Exact Mass: 468.95 g/mol (free acid)
CAS#: 143188-53-8
Purity: ≥ 95 % (HPLC)
Form: solution in water
Color: colorless to slightly yellow
Concentration: 10 mM - 11 mM
pH: 7.5 ±0.5
Spectroscopic Properties: λabs 270 nm, ε 9.5 L mmol-1 cm-1 (Tris-HCl pH 7.5)
Applications:
Monitoring in mononuclear cells by ion-pair LC and MS[1]
Influence on incorporation efficiency[2]
Inhibition of DNA/RNA-dependent DNA-polymerase[3]
Selected References:
[1] Coulier et al. (2008) Simultaneous determination of endogenous deoxynucleotides and phosphorylated nucleoside reverse transcriptase inhibitors in peripheral blood mononuclear cells using ion-pair liquid chromatography coupled to mass spectrometry. Proteomics Clin. Appl. 2:1557.
[2] Lim et al. (2001) Differential incorporation and removal of antiviral deoxynucleotides by human DNA polymerase y. J. Biol. Chem. 276 (26):23616.
[3] Hart et al. (1992) Effects of (-)-2-deoxy-3-thiacytidine (3TC) 5-triphosphate on human immunodeficiency virus reverse transcriptase and mammalian DNA polymerases alpha, beta and gamma. Antimicrobial Agents and Chemotherapy 36 (8):1688.
Boyer et al. (2001) YADD mutants of human immunodeficiency virus type 1 and Moloney murine leukemia virus reverse transcriptase are resistant to lamivudine triphosphate (3TCTP) in vitro. J. Virol. 75:6321.
Feng et al. (1999) Mechanistic studies comparing the incorporation of (+) and (-) isomers of 3TCTP by HIV-1 reverse transcriptase. Biochemistry 38:55.
Kewn et al. (2002) Development of enzymatic assays for quantification of intracellular lamivudine and carbovir triphosphate levels in peripheral blood mononuclear cells from human immunodeficiency virus-infected patients. Antimicrob. Agents Chemother. 46:135.
| Cat. No. | Amount | Price |
|---|---|---|
| NU-1606S | 5 μl (10 mM) | 157,60 € |
| NU-1606L | 5 x 5 μl (10 mM) | 461,30 € |
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Scientific citations for Jena Bioscience nu-1606
5 scientific citations and 2 citation images found by Bioz.
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Discovery of five HIV nucleoside analog reverse-transcriptase inhibitors (NRTIs) as potent inhibitors against the RNA-dependent RNA polymerase (RdRp) of SARS-CoV and 2019-nCoV
bioRxiv, 2020-11-01 · DOI:10.1101/2020.11.01.363788
PrePrint: 6-Mercaptopurine-TP (NU-1110S), 6-chloropurine-TP (NU-1109S), clofarabine-TP (NU-874), 6-methylthio-GTP (NU-1130S), 6-thio-GTP (NU-1106S), stavudine-TP (NU-1604S), 8-oxo-GTP (NU-1116S), gemcitabine-TP (NU-1607S), acyclovir-TP (NU-877), ganciclovir-TP (NU-275S),
lamivudine-TP ( and zidovudine-TP (NU-989S) were purchased fromNU-1606 L)Jena Bioscience . -
Development and evaluation of a simple and effective RT-qPCR inhibitory assay for detection of the efficacy of compounds towards HIV reverse transcriptase.
Applied microbiology and biotechnology, 2018-07-18 · PMID:28963576
Abstract: Assessing the actual efficacy of compounds to directly inhibit HIV reverse transcriptase (RT) activity is a main goal in preclinical antiretroviral studies. Our previous studies demonstrated that the effects of inhibitor compounds towards HIV-RT could be efficiently assessed through a simple cell-free assay based on conventional reverse transcription PCR. In the present study, we describe a modified variant of our assay, termed RT real-time quantitative PCR inhibitory assay (RT-qPCR-IA), in which the ability of compounds to restrict the complementary DNA (cDNA) generation by HIV-RT using a specific RNA template is performed by the real-time technique, in order to improve both accuracy and sensitivity of the method
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Structural basis for the D -stereoselectivity of human DNA polymerase β
Nucleic Acids Research, 2017-06-02 · PMID:28402499
.. was annealed at a 1:1.25:1.15 ratio of upstream primer to downstream primer to template.. The L -nucleotides, L -dCTP, (-)FTC-TP and (-)
3TC-TP were obtained from Jena Bioscience .
