Lamivudine triphosphate, Sodium Salt (L-Isomer)
2', 3'-Dideoxy-3'-thia-cytidine-5'-triphosphate, Sodium salt (L isomer)
For research use only!
Shipping: shipped on blue ice
Storage Conditions: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible.
Shelf Life: 12 months after date of delivery
Molecular Formula: C8H14N3O12P3S (free acid)
Molecular Weight: 469.19 g/mol (free acid)
Exact Mass: 468.95 g/mol (free acid)
Purity: ≥ 95 % (HPLC)
Form: solution in water
Color: colorless to slightly yellow
Concentration: 10 mM - 11 mM
pH: 7.5 ±0.5
Spectroscopic Properties: λabs 270 nm, ε 9.5 L mmol-1 cm-1 (Tris-HCl pH 7.5)
Monitoring in mononuclear cells by ion-pair LC and MS
Influence on incorporation efficiency
Inhibition of DNA/RNA-dependent DNA-polymerase
Please click the black arrow on the right to expand the citation list. Click publication title for the full text.
 Coulier et al. (2008) Simultaneous determination of endogenous deoxynucleotides and phosphorylated nucleoside reverse transcriptase inhibitors in peripheral blood mononuclear cells using ion-pair liquid chromatography coupled to mass spectrometry. Proteomics Clin. Appl. 2:1557.
 Lim et al. (2001) Differential incorporation and removal of antiviral deoxynucleotides by human DNA polymerase y. J. Biol. Chem. 276 (26):23616.
 Hart et al. (1992) Effects of (-)-2-deoxy-3-thiacytidine (3TC) 5-triphosphate on human immunodeficiency virus reverse transcriptase and mammalian DNA polymerases alpha, beta and gamma. Antimicrobial Agents and Chemotherapy 36 (8):1688.
Boyer et al. (2001) YADD mutants of human immunodeficiency virus type 1 and Moloney murine leukemia virus reverse transcriptase are resistant to lamivudine triphosphate (3TCTP) in vitro. J. Virol. 75:6321.
Feng et al. (1999) Mechanistic studies comparing the incorporation of (+) and (-) isomers of 3TCTP by HIV-1 reverse transcriptase. Biochemistry 38:55.
Kewn et al. (2002) Development of enzymatic assays for quantification of intracellular lamivudine and carbovir triphosphate levels in peripheral blood mononuclear cells from human immunodeficiency virus-infected patients. Antimicrob. Agents Chemother. 46:135.