dATP, dCTP, dGTP and dTTP 100 mM solutions
2'-Deoxyadenosine-5'-triphosphate, Sodium salt; 2'-Deoxycytidine-5'-triphosphate, Sodium salt; 2'-Deoxyguanosine-5'-triphosphate, Sodium salt; 2'-Deoxythymidine-5'-triphosphate, Sodium salt
| Cat. No. | Amount | Price (EUR) | Buy / Note |
|---|---|---|---|
| NU-1005S | 4 x 200 μl (4 x 20 μmol) | 78,40 | Add to Basket/Quote Add to Notepad |
| NU-1005L | 4 x 1 ml (4 x 100 μmol) | 312,90 | Add to Basket/Quote Add to Notepad |
For general laboratory use.
Shipping: shipped on gel packs
Storage Conditions: store at -20 °C
Shelf Life: 12 months after date of delivery
Molecular Formula:
dATP: C10H16N5O12P3 (free acid)
dCTP: C9H16N3O13P3 (free acid)
dGTP: C10H16N5O13P3 (free acid)
dTTP: C10H17N2O14P3 (free acid)
Molecular Weight:
dATP: 491.18 g/mol (free acid)
dCTP: 467.15 g/mol (free acid)
dGTP: 507.18 g/mol (free acid)
dTTP: 482.17 g/mol (free acid)
Purity: ≥ 99 % (HPLC)
Form: clear aqueous solution
Concentration: 100 mM - 110 mM
pH: 8.5 ±0.2 (22 °C)
Spectroscopic Properties: dATP: λmax 259 nm, ε 15.1 L mmol-1 cm-1 (Tris-HCl pH 7.0) dCTP: λmax 271 nm, ε 8.9 L mmol-1 cm-1 (Tris-HCl pH 7.0) dGTP: λmax 252 nm, ε 14.2 L mmol-1 cm-1 (Tris-HCl pH 7.0) dTTP: λmax 267 nm, ε 9.5 L mmol-1 cm-1 (Tris-HCl pH 7.0)
Description:
dNTP Bundle contains four separate solutions of ultrapure dATP, dCTP, dGTP and dTTP supplied as clear aqueous solutions (pH 8.5).
| dNTP | Cat. No. | cap color |
| dATP | NU-1001 | red |
| dCTP | NU-1002 | blue |
| dGTP | NU-1003 | yellow |
| dTTP | NU-1004 | green |
Quality Control Specifications:
Low Copy Long Range PCR (18 kb, lambda DNA, template dilution series): PCR fragment with 50 pg of template or less
RT-PCR (749 bp fragment, human GAPDH gene, template dilution series): PCR fragment with 10 pg of template or less
Contamination with bacterial or human DNA: not detectable
DNases, RNases, Nicking Activity: not detectable
Proteases: not detectable
BIOZ Product Citations:
Selected References:
Erlich et al. (1988) Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase. Science 29 (239):487.
Holland et al. (1991) Detection of specific polymerase chain reaction product by utilizing the 5'----3' exonuclease activity of Thermus aquaticus DNA polymerase. Proc. Natl. Acad. Sci. USA 88 (16):7276.
Sanger et al. (1977) DNA sequencing with chain-terminating inhibitors. Proc. Natl. Acad. Sci. USA 74:5463.
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