dPTP
For general laboratory use.
Please centrifuge briefly before opening (volume ≤2 ml).
Shipping: shipped on gel packs
Storage Conditions: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible.
Shelf Life: 12 months after date of delivery
Molecular Formula: C11H18N3O14P3 (free acid)
Molecular Weight: 509.19 g/mol (free acid)
Exact Mass: 509.00 g/mol (free acid)
CAS#: 173964-83-5
Purity: ≥ 95 % (HPLC)
Form: solution in water
Color: colorless to slightly yellow
Concentration: 10 mM - 11 mM
pH: 7.5 ±0.5
Spectroscopic Properties: λmax 294 nm, ε 6.7 L mmol-1 cm-1 (Tris-HCl pH 7.5)
Applications:
Mutagenesis via reverse transcription[1]
Interaction with DNA polymerases alpha and I[2]
Incorporation rate by terminal transferase[3]
Generation of a library of mutants[4]
Selected References:
[1] Petrie et al. (2010) Deep sequencing analysis of mutations resulting from the incorporation of dNTP analogs. Nucleic Acids Res. 38 (22):8095.
[2] Patro et al. (2009) Interaction of human DNA polymerases alpha and DNA polymerase I from Bacillus stearothermophilus with hypoxanthine and 8-oxoguanine nucleotides. Biochemistry48:8271.
[3] Wong et al. (2008) Transversion-enriched sequence saturation mutagenesis (SeSaM-Tv+): a random mutagenesis method with consecutive nucleotide exchanges that complement the bias of error-prone PCR. Biotechnology J.3:74.
[4] Cain et al. (2001) Selection of novel ligands from a whole -molecular randomly mutated C5a library. Protein Engineering14:189.
Huber et al. (2010) Amino acid residues important for folding of thioredoxin are revealed only by study of the physiologically relevant reduced form of the protein. Biochemistry 49 (41):8922.
Cummins et al. (2001) Synthesis and study of the fluorescein conjugate of the nucleotide dPTP. Nucleos. Nucleot. Nucl. 20 (4-7):1049.
Zaccolo et al. (1999) The effect of high-frequency random mutagenesis on in vitro protein evolution: a study on TEM-1 beta-lactamase. Journal of Molecular Biology 285 (2):775.
Zaccolo et al. (1996) An Approach to Random Mutagenesis of DNA Using Mixtures of Triphosphate Derivatives of Nucleoside Analogues. Journal of Molecular Biology 255:589.
Lethal mutagenesis: Loeb, L. et al. (1999) Proc. Natl. Acad. Sci. USA 96:1492.
| Cat. No. | Amount | Price |
|---|---|---|
| NU-1119S | 30 μl (10 mM) | 165,40 € |
| NU-1119L | 5 x 30 μl (10 mM) | 484,60 € |
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