For research use only!
Shipping: shipped at ambient temperature
Storage Conditions: store at 4 °C
Shelf Life: 12 months after date of delivery
Molecular Formula: C18H30N10O3
Molecular Weight: 434.50 g/mol
Exact Mass: 434.25 g/mol
Purity: > 95 % (HPLC)
Form: off-white to grey solid
Solubility: water, DMSO, DMF, MeOH
THPTA is a water-soluble, very effective ligand for Cu(I)-catalyzed Alkyne-Azide click chemistry reactions (CuAAC). It serves a dual purpose: 1) Accelaration of the CuAAC reaction by maintaining the Cu(I) oxidation state of copper sources and 2) Protection of biomolecules from oxidative damage during the labeling reaction[1,2].
THPTA is a superior alternative to water-insoluble TBTA.
A stock solution can be prepared in ddH2O and subsequently be stored at -20°C. Avoid freeze-thaw cycles.
Presolski et al. and Hong et al. provide a general protocol for CuAAC reactions that may be used as a starting point for the set up and optimization of individual assays.
Please click the black arrow on the right to expand the citation list. Click publication title for the full text.
 Presolski et al. (2011) Copper-Catalyzed Azide-Alkyne Click Chemistry for Bioconjugation. Current Protocols in Chemical Biology 3:153.
 Hong et al. (2011) Analysis and Optimization of Copper-Catalyzed Azide-Alkyne Cycloaddition for Bioconjugation. Angew. Chem. Int. Ed. 48:9879.