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Alkyne-containing Fluorescent Dyes

Alkyne-containing Fluorescent dyes can be used for the fluorescent labeling of Azide-tagged molecules via a Cu(I)-catalyzed Azide-Alkyne Click Chemistry reaction (CuAAC).

Standard Dyes (e.g. Cy3, Tamra or Texas Red) have been thoroughly selected to cover the whole UV-Vis spectrum.

 

Table 1: Available Alkyne-containing Fluorescent Dyes

Emission
color
DyeProduct
greenBDP-FL
(also known as BODIPY® FL)
Alkyne - BDP-FL
5-FAM5-FAM-Alkyne
6-FAM6-FAM-Alkyne
AF488
(also known as Alexa Fluor®488)
AF488-Alkyne
5/6-Carboxyrhodamine 1105/6-Carboxyrhodamine 110-PEG4-Alkyne
yellow5-TAMRA5-TAMRA-Alkyne
5/6-TAMRA5/6-TAMRA-PEG4-Alkyne
Sulfo-Cy3Sulfo-Cy3-Alkyne
AF546
(also known as Alexa Fluor®546)
AF546-Alkyne
AF555
(structural analog to Alexa Fluor®555)
AF555-Alkyne
orange5/6-Texas Red5/6-Texas Red-PEG4-Alkyne
AF594
(also known as Alexa Fluor®594)
AF594-Alkyne
redSulfo-Cy5Sulfo-Cy5-Alkyne
AF647
(also known as Alexa Fluor®647)
AF647-Alkyne
Cy5.5Cy5.5-Alkyne
near-IRCy7Cy7-Alkyne