GTPαS

For general laboratory use.

Shipping: shipped on gel packs

Storage Conditions: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible.

Shelf Life: 12 months after date of delivery

Molecular Formula: C₁₀H₁₆N₅O₁₃P₃S (free acid)

Molecular Weight: 539.24 g/mol (free acid)

Exact Mass: 538.97 g/mol (free acid)

CAS#: 81570-51-6

Purity: ≥ 95 % (HPLC)

Form: solution in water

Color: colorless to slightly yellow

Concentration: 100 mM - 110 mM

pH: 7.5 ±0.5

Spectroscopic Properties: λ_max 252 nm, ε 13.7 L mmol^-1 cm^-1 (Tris-HCl pH 7.5)

Applications:
Stability against decapping scavenger pyrophosphatase^[1]
Inhibition of guanylate cyclase^[2]

Specific Ligands:

Translational factor eIF4E^[1]

Bacterial diguanylate cyclase^[3]

BIOZ Product Citations:

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Selected References:
[1] Kowalska et al. (2009) Phosphorothioate analogs of m7GTP are enzymatically stable inhibitors of cap-dependent translation. Bioorganic and Medicinal Chemistry Letters 19:1921.
[2] Garger et al. (2001) Inhibitors of guanylate cyclase inhibit phototransduction in limulus ventral photoreceptors. Visual Neuroscience 18:625.
[3] Wassmann et al. (2007) Structure of BeF3-modified response regulator PleD: implications for diguanylate cyclase activation, catalysis and feedback inhibition. Structure (London) 15:915.
Bao et al. (2008) Coordination of two sequential ester-transfer reactions: exogenous guanosine binding promotes the subsequent wG binding to a group I intron. Nucleic Acids Research 36 (21):6934.
Strobel (1999) A chemogenetic approach to RNA function/structure analysis. Curr. Opin. Struc. Biol. 9 (3):346.
Ryder et al. (1999) Nucleotide analog interference mapping. Methods 18 (1):38.
Antonny et al. (1993) GTP hydrolysis by purified alpha-subunit of transducin and its complex with the cyclic-GMP phosphodiesterase inhibitor. Biochemistry-US 32 (33):8646.