Guanosine-5'-(α-thio)-triphosphate, Sodium salt; (1 : 1 Mixture of Rp and Sp isomers)
For research use only!
Shipping: shipped on blue ice
Storage Conditions: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible.
Shelf Life: 12 months after date of delivery
Molecular Formula: C10H16N5O13P3S (free acid)
Molecular Weight: 539.24 g/mol (free acid)
Exact Mass: 538.97 g/mol (free acid)
Purity: ≥ 95 % (HPLC)
Form: clear aqueous solution
Concentration: 100 mM - 110 mM
pH: 7.5 ±0.5
Spectroscopic Properties: λmax 252 nm, ε 13.7 L mmol-1 cm-1 (Tris-HCl pH 7.5)
Stability against decapping scavenger pyrophosphatase
Inhibition of guanylate cyclase
Translational factor eIF4E
Bacterial diguanylate cyclase
 Kowalska et al. (2009) Phosphorothioate analogs of m7GTP are enzymatically stable inhibitors of cap-dependent translation. Bioorganic and Medicinal Chemistry Letters 19:1921.
 Garger et al. (2001) Inhibitors of guanylate cyclase inhibit phototransduction in limulus ventral photoreceptors. Visual Neuroscience 18:625.
 Wassmann et al. (2007) Structure of BeF3-modified response regulator PleD: implications for diguanylate cyclase activation, catalysis and feedback inhibition. Structure (London) 15:915.
Bao et al. (2008) Coordination of two sequential ester-transfer reactions: exogenous guanosine binding promotes the subsequent wG binding to a group I intron. Nucleic Acids Research 36 (21):6934.
Strobel (1999) A chemogenetic approach to RNA function/structure analysis. Curr. Opin. Struc. Biol. 9 (3):346.
Ryder et al. (1999) Nucleotide analog interference mapping. Methods 18 (1):38.
Antonny et al. (1993) GTP hydrolysis by purified alpha-subunit of transducin and its complex with the cyclic-GMP phosphodiesterase inhibitor. Biochemistry-US 32 (33):8646.