Adenosine-5'-(α-thio)-triphosphate, Sodium salt; (1 : 1 Mixture of Rp and Sp isomers)
For research use only!
Shipping: shipped on gel packs
Storage Conditions: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible.
Shelf Life: 12 months after date of delivery
Molecular Formula: C10H16N5O12P3S (free acid)
Molecular Weight: 523.24 g/mol (free acid)
Exact Mass: 522.97 g/mol (free acid)
Purity: ≥ 95 % (HPLC)
Form: solution in water
Color: colorless to slightly yellow
Concentration: 100 mM - 110 mM
pH: 7.5 ±0.5
Spectroscopic Properties: λmax 259 nm, ε 15.4 L mmol-1 cm-1 (Tris-HCl pH 7.5)
Crystal structure in complex with adenylyl cyclase
Activation of beta-cell adenylyl cyclase
Potent agonist for P2Y11-receptor
Full agonist for rP2Y1-receptor
Binding to P2Y2- and P2Y6-receptors
Ligand for purinergic receptors:
BIOZ Product Citations:
Please click the arrow on the right to expand the citation list. Click publication title for the full text.
 Mou et al. (2009) Sructural basis for inhibition of mammalian adenylyl cyclase by calcium. Biochemistry 48 (15):3387.
 Chevassus et al. (2002) P2Y receptor activation enhances insulin release from pancreatic beta-cells by triggering the cyclic AMP/protein kinase A pathway. Naunyn Schmiedebergs Arch. Pharmacol. 366 (5):464.
 Van der Weyden et al. (2000) Pharmacological characterisation of of P2Y11 receptor in stably transfected haematological cell lines. Mol. Cell. Biochem. 213 (1):75.
 Vohringer et al. (2000) A chimeric rat brain P2Y1 receptor tagged with GFP: high-affinity ligand recognition of adenosine diphosphates and triphosphates and selectivity identical to that of wild-type receptor. Biochem. Pharmacol. 59 (7):791.
 Schafer et al. (2003) ATP- and UTP-activated P2Y receptors differently regulate proliferation of human lung epithelial tumor cells. Am. J. Physiol. Lung Cell. Mol. Physiol. 285 (2):L376.
 Gorodeski (2002) Regulation of transcervical permeability by two distinct P2 purinergic receptor mechanisms. Am. J. Physiol. Cell Physiol. 282 (1):C75.
 Schäfer et al. (1999) ATPalphaS is a ligand for P2Y receptors in synaptosomal membranes: solubilization of[35S]ATPalphaS binding proteins associated with G-proteins. Neurochemistry Int. 34 (4):303.
Kim et al. (2009) Profiling the selectivity of DNA ligases in an array format with mass spectrometry. Nucleic Acids Research 38 (1):e2.
Vohringer et al. (2001) Pharmacological characterization of the rat brain P2Y (1) receptor expressed in HEK293 cells: Ca2+ signaling and receptor regulation. Drug Develop. Res. 53 (2):172.
Ryder et al. (1999) Nucleotide analog interference mapping. Methods 18 (1):38.
OsumiDavis et al. (1996) Effect of alpha-Thio-ATP on the steady-state kinetics of nucleotide incorporation catalyzed by the wildtype and active-site mutant T7 RNA polymerases. Biophys. J. 70 (2):SU480, Part 2.
Cristalli et al. (1993) Identification of a receptor for ADP on blood-platelets by photoaffinity-labeling. Biochem. J. 291:875, Part 3.
Savi et al. (1993) ADP-dependence of platelet activation-induced by a thrombin receptor agonist. Nouv. Rev. Fr. Hematol. 35 (2):115.
Shi et al. (1993) Mechanism of adenylate kinase - what can be learned from a mutant enzyme with minor perturbation in kinetic-parameters. Biochemistry-US 32 (25):6450.
Cullis et al. (1992) Energy coupling in DNA gyrase - a thermodynamic limit to the extent of DNA supercoiling. Biochemistry- US 31 (40):9642.
Tomkinson et al. (1992) DNA ligase-I from Saccharomyces-cerevisiae - physical and biochemical characterization of the cdc9 gene-product. Biochemistry-US 31 (47):11762.