Adenosine-5'-(α-thio)-triphosphate, Sodium salt; (1 : 1 Mixture of Rp and Sp isomers)
For research use only!
Shipping: shipped on blue ice
Storage Conditions: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible.
Shelf Life: 12 months after date of delivery
Molecular Formula: C10H16N5O12P3S (free acid)
Molecular Weight: 523.24 g/mol (free acid)
Purity: ≥ 95 % (HPLC)
Form: clear aqueous solution
Concentration: 100 mM - 110 mM
pH: 7.5 ±0.5
Spectroscopic Properties: λmax 259 nm, ε 15.4 L mmol-1 cm-1 (Tris-HCl pH 7.5)
Crystal structure in complex with adenylyl cyclase
Activation of beta-cell adenylyl cyclase
Potent agonist for P2Y11-receptor
Full agonist for rP2Y1-receptor
Binding to P2Y2- and P2Y6-receptors
Ligand for purinergic receptors:
 Mou et al. (2009) Sructural basis for inhibition of mammalian adenylyl cyclase by calcium. Biochemistry 48 (15):3387.
 Chevassus et al. (2002) P2Y receptor activation enhances insulin release from pancreatic beta-cells by triggering the cyclic AMP/protein kinase A pathway. Naunyn Schmiedebergs Arch. Pharmacol. 366 (5):464.
 Van der Weyden et al. (2000) Pharmacological characterisation of of P2Y11 receptor in stably transfected haematological cell lines. Mol. Cell. Biochem. 213 (1):75.
 Vohringer et al. (2000) A chimeric rat brain P2Y1 receptor tagged with GFP: high-affinity ligand recognition of adenosine diphosphates and triphosphates and selectivity identical to that of wild-type receptor. Biochem. Pharmacol. 59 (7):791.
 Schafer et al. (2003) ATP- and UTP-activated P2Y receptors differently regulate proliferation of human lung epithelial tumor cells. Am. J. Physiol. Lung Cell. Mol. Physiol. 285 (2):L376.
 Gorodeski (2002) Regulation of transcervical permeability by two distinct P2 purinergic receptor mechanisms. Am. J. Physiol. Cell Physiol. 282 (1):C75.
 Schäfer et al. (1999) ATPalphaS is a ligand for P2Y receptors in synaptosomal membranes: solubilization of[35S]ATPalphaS binding proteins associated with G-proteins. Neurochemistry Int. 34 (4):303.
Kim et al. (2009) Profiling the selectivity of DNA ligases in an array format with mass spectrometry. Nucleic Acids Research 38 (1):e2.
Vohringer et al. (2001) Pharmacological characterization of the rat brain P2Y (1) receptor expressed in HEK293 cells: Ca2+ signaling and receptor regulation. Drug Develop. Res. 53 (2):172.
Ryder et al. (1999) Nucleotide analog interference mapping. Methods 18 (1):38.
OsumiDavis et al. (1996) Effect of alpha-Thio-ATP on the steady-state kinetics of nucleotide incorporation catalyzed by the wildtype and active-site mutant T7 RNA polymerases. Biophys. J. 70 (2):SU480, Part 2.
Cristalli et al. (1993) Identification of a receptor for ADP on blood-platelets by photoaffinity-labeling. Biochem. J. 291:875, Part 3.
Savi et al. (1993) ADP-dependence of platelet activation-induced by a thrombin receptor agonist. Nouv. Rev. Fr. Hematol. 35 (2):115.
Shi et al. (1993) Mechanism of adenylate kinase - what can be learned from a mutant enzyme with minor perturbation in kinetic-parameters. Biochemistry-US 32 (25):6450.
Cullis et al. (1992) Energy coupling in DNA gyrase - a thermodynamic limit to the extent of DNA supercoiling. Biochemistry- US 31 (40):9642.
Tomkinson et al. (1992) DNA ligase-I from Saccharomyces-cerevisiae - physical and biochemical characterization of the cdc9 gene-product. Biochemistry-US 31 (47):11762.