ITP

For general laboratory use.

Please centrifuge briefly before opening (volume ≤2 ml).

Shipping: shipped on gel packs

Storage Conditions: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible.

Shelf Life: 12 months after date of delivery

Molecular Formula: C₁₀H₁₅N₄O₁₄P₃ (free acid)

Molecular Weight: 508.16 g/mol (free acid)

Exact Mass: 507.98 g/mol (free acid)

CAS#: 35908-31-7

Purity: ≥ 95 % (HPLC)

Form: solution in water

Color: colorless to slightly yellow

Concentration: 100 mM - 110 mM

pH: 7.5 ±0.5

Spectroscopic Properties: λ_max 249 nm, ε 12.2 L mmol^-1 cm^-1 (Tris-HCl pH 7.5)

Specific Ligands:

Specific and agonistic ligand for P2Y₄ receptor^[1]

Selected References:
[1] Vial and Evans (2002) P2X1 receptor-deficient mice establish the native P2X receptor and P2Y6-like receptor in arteries. Molec. Pharmac. 62 (6):1438.
Bao et al. (2008) Coordination of two sequential ester-transfer reactions: exogenous guanosine binding promotes the subsequent wG binding to a group I intron. Nucleic Acids Research 36 (21):6934.
Chang et al. (2005) Nitric Oxide-dependent Allosteric Inhibitory Role of a Second Nucleotide Binding Site in Soluble Guanylyl Cyclase. J. Biol. Chem. 280 (12):11513.
Bianchi et al. (2001) Intramolecular equilibria in metal ion complexes of guanosine 5 '-triphosphate (GTP (4-)) and inosine 5 '-triphosphate (ITP4-) in aqueous solution. J. Inorg. Biochem. 86 (1):148.
Noji et al. (2001) Purine but not pyrimidine nucleotides support rotation of F (1)-ATPase. J. Biol. Chem. 276 (27):25480.
Chakrabarti et al. (2000) Nucleoside triphosphate specificity of tubulin. Biochemistry 39 (33):10269.
Jacob et al. (2000) Involvement of asparagine 118 in the nucleotide specificity of the catalytic subunit of protein kinase CK2. FEBS Lett. 466 (2-3):363.
Seifert et al. (1999) Effects of guanine, inosine, and xanthine nucleotides on beta (2)-adrenergic receptor/G (s) interactions: evidence for multiple receptor conformations. Mol. Pharmacol. 56 (2):348.
Wang et al. (1999) Identification of residues of Escherichia coli phosphofructokinase that contribute to nucleotide binding and specificity. Biochemistry 38 (14):4313.
Nakahara et al. (1998) Inosine 5 '-triphosphate can dramatically increase the yield of NASBA products targeting GC-rich and intramolecular base-paired viroid RNA. Nucleic Acids Res. 26 (7):1854.
Sasaki et al. (1998) Identification of stable RNA hairpins causing band compression in transcriptional sequencing and their elimination by use of inosine triphosphate. Gene 222 (1):17.
Klinker et al. (1997) Functionally nonequivalent interactions of guanosine 5'-triphosphate, inosine 5'-triphosphate, and xanthosine 5'- triphosphate with the retinal G-protein, transducin, and with G (i)-proteins in HL-60 leukemia cell membranes. Biochem. Pharmacol. 54 (5):551.
Pollardknight et al. (1987) Kinetics of Hexokinase-D (Glucokinase) with inosine triphosphate as phosphate donor - loss of kinetic cooperativity with respect to glucose. Biochem. J. 245 (3):625.
Chanda et al. (1983) Invitro synthesis of genome length complementary RNA of vesicular stomatitis-virus in the presence of inosine 5'-triphosphate. Virology 129 (1):225.
Morgan et al. (1980) Initiation of reovirus transcription by inosine 5'-triphosphate and properties of 7-methylinosine-capped, inosinesubstituted messenger ribonucleic-acids. Biochemistry-US 19 (26):5960.
West (1970) Adenosine triphosphate and inosine triphosphate dependent conformational changes of adenosine diphosphate-G-actin. Biochemistry-US 9 (20):3847.
Azuma et al. (1963) Kinetic studies on hydrolysis of adenosine triphosphate and inosine triphosphate by Myosin A. Biochim. Biophys. Acta 73 (3):499.