N6-Propargyl-adenosine-5'-triphosphate, Sodium salt
For research use only!
Shipping: shipped on blue ice
Storage Conditions: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible.
Shelf Life: 12 months after date of delivery
Molecular Formula: C13H18N5O13P3 (free acid)
Molecular Weight: 545.23 g/mol (free acid)
Purity: ≥ 95 % (HPLC)
Form: white to off-white solid
Solubility: 10 mM Tris-HCl pH 7.5
Spectroscopic Properties: λmax 262 nm, ε 18.0 L mmol-1 cm-1 (Tris-HCl pH 7.5)
in vitro AMPylation of proteins[1,2]
in vitro polyadenylation of RNA
The resulting alkyne-functionalized protein[1,2] or RNA can subsequently be processed via Cu(I)-catalyzed (azide-alkyne) click chemistry that offers the choice
Please note: This compound contains a phosphoramide linkage which is hydrolyzed at pH <7.0.
For preparation of a 10 mM solution use 100 mM buffer (for example: bicarbonate, borate, phosphate and Tris) to prevent degradation at acidic pH.
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 Grammel et al. (2012) Chemical Reporter for Monitoring RNA Synthesis and Poly (A) Tail Dynamics. ChemBioChem 13:1112.
 Presolski et al. (2011) Copper-Catalyzed Azide-Alkyne Click Chemistry for Bioconjugation. Current Protocols in Chemical Biology 3:153.
 Hong et al. (2011) Analysis and Optimization of Copper-Catalyzed Azide-Alkyne Cycloaddition for Bioconjugation. Angew. Chem. Int. Ed. 48:9879.
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