GpCpp

For general laboratory use.

Please centrifuge briefly before opening (volume ≤2 ml).

Shipping: shipped on gel packs

Storage Conditions: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible.

Shelf Life: 12 months after date of delivery

Molecular Formula: C₁₁H₁₈N₅O₁₃P₃ (free acid)

Molecular Weight: 521.21 g/mol (free acid)

Exact Mass: 521.01 g/mol (free acid)

CAS#: 14997-54-7

Purity: ≥ 95 % (HPLC)

Form: solution in water

Color: colorless to slightly yellow

Concentration: 10 mM - 11 mM

pH: 7.5 ±0.5

Spectroscopic Properties: λ_max 252 nm, ε 13.7 L mmol^-1 cm^-1 (Tris-HCl pH 7.5)

Applications:
Atomic force microscopy^[1]
Cryomicroscopy^[2]
Assembly of microtubule^[3]
Dynamic of microtubule bundles^[4]

Specific Ligands:

Tubulin^[5]

GTP cyclohydrolase^[6]

Selected References:
[1] Thomson et al. (2003) Large fluctuations in the disassembly rate of microtubules revealed by atomic force microscopy. Ultramicroscopy 97:239.
[2] Meurer-Grob et al. (2001) Microtubule structure at improved resolution. Biochemistry-US 40 (27):8000.
[3] Dixit et al. (2009) Microtubule plus-end tracking by CLIP-170 requires EB1. PNAS USA 106:492.
[4] Laan et al. (2008) Force-generation and dynamic instability of microtubule bundies. PNAS USA 105:8920.
[5] Shanker et al. (2007) Enhanced microtubule binding and tubulin assembly properties of conformationally constraint Paclitaxel derivatives. Biochemistry 46:11514.
[6] Ren et al. (2005) GTP cyclohydrolase II structure and mechanism. J. Biol. Chem. 280:36912.
Franck et al. (2010) Direct physical study of kinetochore-microtubule interactions by reconstitution and interrogation with an optical force clamp. Methods 51 (2):242.
Nitzsche et al. (2010) Studying kinesin motors by optical 3D-nanometry in gliding motility assays. Methods Cell. Biol. 95:247.
Gell et al. (2010) Microtubule dynamics reconstituted in vitro and imaged by single-molecule fluorescence microscopy. Methods Cell. Biol. 95:221.
Peters et al. (2010) Insight into the molecular mechanism of the multitasking kinesin-8 motor. EMBO J. 29 (20):3437.
Khrapunovich-Baine et al. (2009) Distinct Pose of Discodermolide in Taxol Binding Pocket Drives a Complementary Mode of Microtubule Stabilization. Biochemistry 48 (49):11677.
Nitzsche, et al. (2009) Quantum-dot-assisted characterization of microtubule rotations during cargo transport. Nature Nanotechnology 3:553.
Asbury et al. (2007) Tension applied through the Dam1 complex promotes microtubule elongation providing a direct mechanism for length control in mitosis. Nature Cell Biology 9:832.
Cary et al. (2005) Tonic and acute nitric oxide signaling through soluble guanylate cyclase is mediated by nonheme nitric oxide, ATP, and GTP. Nature Structural & Molecular Biology 102 (37) :13064.
Klaholz et al. (2005) Conformational transition of initiation factor 2 from the GTP- to GDP- bound state visualized on the ribosome. Nature Structural & Molecular Biology 11 :1145.
Li et al. (2000) Equilibrium studies of a fluorescent paclitaxel derivative binding to microtubules. Biochemistry-US 39 (3):616.
Lowe et al. (2000) Helical tubes of ftsz from Methanococcus jannaschii. Biol. Chem. 381 (9-10):993.
Muller-Reichert et al. (1998) Structural changes at microtubule ends accompanying GTP hydrolysis: information from a slowly hydrolyzable analogue of GTP, guanylyl (alpha,beta)methylenediphosphonate. Proc. Natl. Acad. Sci. USA 95 (7):3661.
Tran et al. (1997) A metastable intermediate state of microtubule dynamic instability that differs significantly between plus and minus ends. J. Cell Biol. 138 (1):105.
Tran et al. (1997) How tubulin subunits are lost from the shortening ends of microtubules. J. Struct. Biol. 118 (2):107.
Vulevic et al. (1997) Role of guanine nucleotides in the vinblastine-induced self-association of tubulin: effects of guanosine alpha,betamethylenetriphosphate and guanosine alpha,beta-methylenediphosphate. Biochemistry-US 36 (42):12828.
Caplow et al. (1996) Evidence that a single monolayer tubulin-GTP cap is both necessary and sufficient to stabilize microtubules. Mol. Biol. Cell 7 (4):663.
Hyman et al. (1995) Structural-changes accompanying GTP hydrolysis in microtubules - information from a slowly hydrolyzable analog guanylyl- (alpha,beta)-methylene-diphosphonate. J. Cell Biol. 128 (1-2):117.