2'/3'-O-(N-Methyl-anthraniloyl)-guanosine-5'-diphosphate, Triethylammonium salt
For research use only!
Shipping: shipped on blue ice
Storage Conditions: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible.
Shelf Life: 12 months after date of delivery
Molecular Formula: C18H22N6O12P2 (free acid)
Molecular Weight: 576.35 g/mol (free acid)
Exact Mass: 576.08 g/mol (free acid)
Purity: ≥ 95 % (HPLC)
Form: colorless to slightly yellow solution in water
Concentration: 10 mM - 11 mM
pH: 7.5 ±0.5
Spectroscopic Properties: λmax 252/355 nm, ε 22.6/5.7 L mmol-1 cm-1 (Tris-HCl pH 7.5), λexc 355 nm, λem 448 nm
Conformational dynamic: DnaB/C-proteins, Csk
Fluorescence stop-flow kinetics: release factor, elF5B, EF-1B, EF-Tu
Translation factor: elF5B, EF1B2, EF-Tu
Please click the black arrow on the right to expand the citation list. Click publication title for the full text.
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Oreb et al. (2011) Substrate binding disrupts dimerization and induces nucleotide exchange of the chloroplast GTPase Toc33.. Biochem J. 436 (2):313.
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Yang et al. (2010) A guaninine nucleotide exchange factor is a component of the meiotic spindle pole body in Schizosaccharomyces pombe. Mol. Biol. Cell. 21 (7):1272.
Dahl et al. (2008) The Importance of P-loop and Domain Movements in EF-Tu for Guanine Nucleotide Exchange. J. Biol. Chem. 281 (30):21139.
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Noel et al. (2007) Turning off the Ras switch with the flick of a finger. Nature Structural & Molecular Biology 4:677.
Pestova et al. (2006) Kinetic Analysis of Interaction of Eukaryotic Release Factor 3 with Guanine Nucleotides. J. Biol. Chem. 281 (52):40224.
Pick et al. (2006) Liposomes Comprising Anionic but not neutral Phospholipids cause dissociation of Rac (1 or 2)RhoGDI Complexes and support Amphiphile-independent NADPH oxidase Activation by Such Complexes . J. Biol. Chem. 281 (28):19204.
Wintermeyer et al. (2006) Role and timing of GTP binding and hydrolysis during EF-G-dependent RNA translocation on the ribosome. PNAS 103 (37):13670.
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Ahmadian et al. (1997) Individual rate constants for the interaction of Ras proteins with GTPase-activating proteins determined by fluorescence spectroscopy. Biochemistry 36:4535.
Remmers et al. (1996) Partial G protein activation by fluorescent guanine nucleotide analogs. Evidence for a triphosphatebound but inactive state. J. Biol. Chem. 271:4791.
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Neal et al. (1990) Hydrolysis of GTP by p21NRAS, the NRAS protooncogene product, is accompanied by a conformational change in the wild-type protein: use of a single fluorescent probe at the catalytic site. Proc. Natl. Acad. Sci. USA 87:3562.