A hallmark of cancer is uncontrolled cell proliferation caused by deregulation of multiple signaling pathways associated with cell replication, metabolism and programmed cell death.
Cytostatic nucleotides are substrate analogs of physiological nucleotides that inhibit cell proliferation by interfering with cellular targets involved in DNA/RNA synthesis and nucleotide metabolism.
In vivo or cell culture experiments can be performed using the non-phosphorylated nucleoside variant of a cytostatic nucleotide that is metabolized by multiple cellular kinases to its active phosphorylated forms (NMP → NDP → NTP).
Alternatively, a number of mono-, di- and triphosphorylated analogs of cytostatic nucleotides are available for functional in vitro experiments (Tab. 1).
Please refer to the corresponding data sheet for detailed application data.
 Hanahan et al. (2011) Hallmarks of Cancer: The next generation. Cell 144 (5):646.