5-Aza-dCTP

For general laboratory use.

Description:
5-Aza-dCTP is a inhibitor of DNA methylation and an antileukemic agent. It has been used to reactivate silent tumor suppressor genes.

Shipping: shipped on gel packs

Storage Conditions: store at -20 °C

Shelf Life: 12 months after date of delivery

Molecular Formula: C₈H₁₅N₄O₁₃P₃ (free acid)

Molecular Weight: 468.14 g/mol (free acid)

Exact Mass: 467.98 g/mol (free acid)

CAS#: 72052-96-1

Purity: ≥ 95 % (HPLC)

Form: solid

Spectroscopic Properties: λ_max 244 nm, ε 7.0 L mmol^-1 cm^-1 (Tris-HCl pH 7.5)

Applications:
Determination of methyltransferase activity^[1]

Specific Ligands:

DNA-binding^[1]

Please note:

1.) Contains at least 10 mg product

2.) The triazine moiety of decitabine is prone to acid- and base-catalyzed decomposition.^[2] Always prepare a fresh solution for immediate use. Neutral buffers and low temperatures are most appropriate.

Selected References:
[1] Frauer et al. (2009) A versatile non-radioactive assay for DNA methyltransferase activity and DNA binding. Nucleic Acids Research 37 (3):e22.
[2] Lin et al. (1981) High-performance liquid chromatographic analysis of chemical stability of 5-aza-2'-deoxycytidine J. Pharm. Sci. 70 (11):1228.
Lemaire et al. (2005) Enhancement of antineoplastic action of 5-aza-2'-deoxycytidine by zebularine on L1210 leukemia. Anticancer Drugs. 16 (3):301.
Parker et al. (1987) Inhibition of DNA primase by nucleoside triphosphates and their arabinofuranosyl analogs. Mol. Pharmacol. 31 (2):146.
McIntosh et al. (1985) Synthesis and characterization of poly[d (G-z5C)]. B-Z transition and inhibition of DNA methylase. Biochemistry 24 (18):4806.
Momparler et al. (1984) Kinetic interaction of 5-AZA-2'-deoxycytidine-5'-monophosphate and its 5'-triphosphate with deoxycytidylate deaminase. Mol. Pharmacol. 25 (3):436.
Bouchard et al. (1983) Incorporation of 5-Aza-2'-deoxycytidine-5'-triphosphate into DNA. Interactions with mammalian DNA polymerase alpha and DNA methylase. Mol. Pharmacol. 24 (1):109.
Bouchard et al. (1983) Incorporation of 5-Aza-2'-deoxycytidine-5'-triphosphate into DNA. Interactions with mammalian DNA polymerase alpha and DNA methylase. Mol. Pharmacology 24 (1):109.