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5-Aza-dCTP

Decitabine triphosphate

5-Aza-2'-deoxycytidine-5'-triphosphate, Sodium salt

Cat. No. Amount Price (EUR) Buy / Note
NU-1118 20 mg 1.588,98 Add to Basket/Quote Add to Notepad
Structural formula of 5-Aza-dCTP (Decitabine triphosphate, 5-Aza-2'-deoxycytidine-5'-triphosphate, Sodium salt)
Structural formula of 5-Aza-dCTP

For research use only!

Shipping: shipped on blue ice

Storage Conditions: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible.

Shelf Life: 12 months after date of delivery

Molecular Formula: C8H15N4O13P3 (free acid)

Molecular Weight: 468.14 g/mol (free acid)

Exact Mass: 467.98 g/mol (free acid)

CAS#: 72052-96-1

Purity: ≥ 95 % (HPLC at 267 nm)

Form: solid

Spectroscopic Properties: λmax 244 nm, ε 7.0 L mmol-1 cm-1 (Tris-HCl pH 7.5)

Applications:
Determination of methyltransferase activity[1]

Description:
5-Aza-dCTP is a inhibitor of DNA methylation and an antileukemic agent. It has been used to reactivate silent tumor suppressor genes.

Specific Ligands:

DNA-binding[1]

Please note:

1.) Contains at least 10 mg product

2.) The triazine moiety of decitabine is prone to acid- and base-catalyzed decomposition.[2] To assure highest quality standards, the compound will be freshly prepared for you and shipped as a solid. Due to the limited stability, the price is higher than for stable nucleotides which can be kept in stock. We strongly suggest always to prepare fresh solutions for immediate use. For this, neutral buffers and low temperatures are most appropriate.

Product Citations:
Please click the black arrow on the right to expand the citation list. Click publication title for the full text.

Selected References:
[1] Frauer et al. (2009) A versatile non-radioactive assay for DNA methyltransferase activity and DNA binding. Nucleic Acids Research 37 (3):e22.
[2] Lin et al. (1981) High-performance liquid chromatographic analysis of chemical stability of 5-aza-2'-deoxycytidine J. Pharm. Sci. 70 (11):1228.
Lemaire et al. (2005) Enhancement of antineoplastic action of 5-aza-2'-deoxycytidine by zebularine on L1210 leukemia. Anticancer Drugs. 16 (3):301.
Parker et al. (1987) Inhibition of DNA primase by nucleoside triphosphates and their arabinofuranosyl analogs. Mol. Pharmacol. 31 (2):146.
McIntosh et al. (1985) Synthesis and characterization of poly[d (G-z5C)]. B-Z transition and inhibition of DNA methylase. Biochemistry 24 (18):4806.
Momparler et al. (1984) Kinetic interaction of 5-AZA-2'-deoxycytidine-5'-monophosphate and its 5'-triphosphate with deoxycytidylate deaminase. Mol. Pharmacol. 25 (3):436.
Bouchard et al. (1983) Incorporation of 5-Aza-2'-deoxycytidine-5'-triphosphate into DNA. Interactions with mammalian DNA polymerase alpha and DNA methylase. Mol. Pharmacol. 24 (1):109.
Bouchard et al. (1983) Incorporation of 5-Aza-2'-deoxycytidine-5'-triphosphate into DNA. Interactions with mammalian DNA polymerase alpha and DNA methylase. Mol. Pharmacology 24 (1):109.