For research use only!
Shipping: shipped at ambient temperature
Storage Conditions: store at -20 °C
store undissolved, for use prepare a fresh solution
Shelf Life: 12 months after date of delivery (undissolved)
Molecular Formula: C39H51N5O8S
Molecular Weight: 749.92 g/mol
Purity: > 90 % (HPLC)
Form: off-white to slightly yellow solid at storage conditions, tends to become sticky amorphous solid
Solubility: DMF, DMSO
DBCO-PEG4-Biotin is suitable for the introduction of a biotin moiety to Azide-labeled biomolecules via Cu(I)-free strain-promoted Alkyne-Azide Click Chemistry (SPAAC) reaction.
The hydrophilic PEG4 linker [22.8 Å (19 atoms)] reduces or eliminates aggregation and precipitation during the labeling process by increasing the hydrophilicity of the target molecule. It furthermore enhances the accessibility of the biotin moiety and thus the detection efficiency of the biotinylated molecule via fluorescent or HRP-labeled streptavidin or its affinity purification via streptavidin agarose.
Due to the extremely high affinity of biotin towards streptavidin (KD = 10-15 M), the biotinylated molecule/streptavidin-interaction is essentially irreversible under physiological conditions.
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