» Sign in / Register

DBCO-PEG4-Biotin Conjugate

Dibenzylcyclooctyne-PEG4-Biotin Conjugate

Cat. No. Amount Price (EUR) Buy / Note
CLK-A105P4-10 10 mg 158,35 Add to Basket/Quote Add to Notepad
CLK-A105P4-100 10 x 10 mg 1.028,91 Add to Basket/Quote Add to Notepad
Structural formula of DBCO-PEG4-Biotin Conjugate
Structural formula of DBCO-PEG4-Biotin Conjugate

For research use only!

Shipping: shipped at ambient temperature

Storage Conditions: store at -20 °C
store undissolved, for use prepare a fresh solution

Shelf Life: 12 months after date of delivery (undissolved)

Molecular Formula: C39H51N5O8S

Molecular Weight: 749.92 g/mol

CAS#: 1255942-07-4

Purity: > 90 % (HPLC)

Form: off-white to slightly yellow solid at storage conditions, tends to become sticky amorphous solid

Solubility: DMF, DMSO

Description:
DBCO-PEG4-Biotin is suitable for the introduction of a biotin moiety to Azide-labeled biomolecules via Cu(I)-free strain-promoted Alkyne-Azide Click Chemistry (SPAAC) reaction.

The hydrophilic PEG4 linker [22.8 Å (19 atoms)] reduces or eliminates aggregation and precipitation during the labeling process by increasing the hydrophilicity of the target molecule. It furthermore enhances the accessibility of the biotin moiety and thus the detection efficiency of the biotinylated molecule via fluorescent or HRP-labeled streptavidin or its affinity purification via streptavidin agarose.

Due to the extremely high affinity of biotin towards streptavidin (KD = 10-15 M), the biotinylated molecule/streptavidin-interaction is essentially irreversible under physiological conditions.

Product Citations:
Please click the black arrow on the right to expand the citation list. Click publication title for the full text.