Dibenzocyclooctyne (DBCO)*-containing fluorescent dyes can be used for the fluorescent labeling of Azide-tagged biomolecules via Cu(I)-free strain-promoted Alkyne-Azide Click Chemistry (SPAAC). Both Standard Dyes (e.g. Cy3, Tamra or Texas Red) and novel Alternative Dyes have been thoroughly selected to cover the whole UV-Vis spectrum.
*DBCO is also known as ADIBO (= Azadibenzocyclooctyne)
Emission color |
Dye | Product |
---|---|---|
blue-green | ATTO 425 | DBCO-PEG4-ATTO-425 |
green | BDP-FL | BDP-FL-PEG4-DBCO |
5/6-Fluorescein (5/6-FAM) | DBCO-PEG4-5/6-FAM | |
AZDye 488 | DBCO-AZDye488 | |
ATTO 488 | DBCO-PEG4-ATTO-488 | |
5/6-Carboxyrhodamine 110 | DBCO-PEG4-5/6-Carboxyrhodamine 110 | |
yellow | 5-TAMRA | DBCO-PEG4-5-TAMRA |
Sulfo-Cy3 | DBCO-Sulfo-Cy3 | |
AZDye 546 | DBCO-AZDye546 | |
AZDye 555 | DBCO-AZDye555 | |
orange | AZDye 594 | DBCO-AZDye594 |
red | Sulfo-Cy5 | DBCO-Sulfo-Cy5 |
AZDye 647 | DBCO-AZDye647 | |
Cy5.5 | DBCO-Cy5.5 |