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DBCO-containing Fluorescent Dyes

Dibenzocyclooctyne (DBCO)*-containing fluorescent dyes can be used for the fluorescent labeling of Azide-tagged biomolecules via Cu(I)-free strain-promoted Alkyne-Azide Click Chemistry (SPAAC). Both Standard Dyes (e.g. Cy3, Tamra or Texas Red) and novel Alternative Dyes have been thoroughly selected to cover the whole UV-Vis spectrum.

*DBCO is also known as ADIBO (= Azadibenzocyclooctyne)

 

Table 1: Available DBCO-containing Fluorescent Dyes

Emission
color
Dye Product
blue-green ATTO 425 DBCO-PEG4-ATTO-425
green BDP-FL
(also known as BODIPY® FL)
BDP-FL-PEG4-DBCO
5/6-Fluorescein (5/6-FAM) DBCO-PEG4-5/6-FAM
AF488
(also known as Alexa Fluor®488)
DBCO-AF488
ATTO 488 DBCO-PEG4-ATTO-488
5/6-Carboxyrhodamine 110 DBCO-PEG4-5/6-Carboxyrhodamine 110
yellow 5/6-TAMRA DBCO-PEG4-5/6-TAMRA
Sulfo-Cy3 DBCO-Sulfo-Cy3
AF546
(also known as Alexa Fluor®546)
DBCO-AF546
AF555
(structural analog to Alexa Fluor®555)
DBCO-AF555
orange AF594
(also known as Alexa Fluor®594)
DBCO-AF594
red Sulfo-Cy5 DBCO-Sulfo-Cy5
AF647
(structural analog to Alexa Fluor®647)
DBCO-AF647
Cy5.5 DBCO-Cy5.5