Dibenzocyclooctyne (DBCO)*-containing fluorescent dyes can be used for the fluorescent labeling of Azide-tagged biomolecules via Cu(I)-free strain-promoted Alkyne-Azide Click Chemistry (SPAAC). Both Standard Dyes (e.g. Cy3, Tamra or Texas Red) and novel Alternative Dyes have been thoroughly selected to cover the whole UV-Vis spectrum.
*DBCO is also known as ADIBO (= Azadibenzocyclooctyne)
| Emission color |
Dye | Product |
|---|---|---|
| blue-green | ATTO 425 | DBCO-PEG4-ATTO-425 |
| green | BDP-FL (also known as BODIPY® FL) |
BDP-FL-PEG4-DBCO |
| 5/6-Fluorescein (5/6-FAM) | DBCO-PEG4-5/6-FAM | |
| AF488 (also known as Alexa Fluor®488) |
DBCO-AF488 | |
| ATTO 488 | DBCO-PEG4-ATTO-488 | |
| 5/6-Carboxyrhodamine 110 | DBCO-PEG4-5/6-Carboxyrhodamine 110 | |
| yellow | 5-TAMRA | DBCO-PEG4-5-TAMRA |
| Sulfo-Cy3 | DBCO-Sulfo-Cy3 | |
| AF546 (also known as Alexa Fluor®546) |
DBCO-AF546 | |
| AF555 (structural analog to Alexa Fluor®555) |
DBCO-AF555 | |
| orange | AF594 (also known as Alexa Fluor®594) |
DBCO-AF594 |
| red | Sulfo-Cy5 | DBCO-Sulfo-Cy5 |
| AF647 (structural analog to Alexa Fluor®647) |
DBCO-AF647 | |
| Cy5.5 | DBCO-Cy5.5 |
