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DBCO-containing FLAG Reagents

Dibenzocyclooctyne (DBCO)*-containing FLAG tag reagents can be used for the introduction of a FLAG-tag to Azide-tagged biomolecules via Cu(I)-free strain-promoted Alkyne-Azide Click Chemistry (SPAAC). Molecule solubility and efficient FLAG-tag detection is ensured by the integrated PEG-linker.

*DBCO is also known as ADIBO (= Azadibenzocyclooctyne) or DIBAC (= Dibenzoazacyclooctyne)


Selected References

[1] Hopp et al. (1988) A Short Polypeptide Marker Sequence Useful for Recombinant Protein Identification and Purification. Bio/Technology 6:1204.
[2] Einhauer et al. (2001) The FLAG peptide, a versatile fusion tag for the purification of recombinant proteins. J. Biochem. Biophys. Methods 49:455.
[3] Brizzard et al. (1997) Epitope tagging of recombinant proteins. Current Protocols in Neuroscience:5.8.1.