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DBCO-containing (Desthio) Biotinylation Reagents

Dibenzocyclooctyne (DBCO)*-containing (desthio) biotinylation reagents can be used for the labeling of Azide-tagged biomolecules via Cu(I)-free strain-promoted Alkyne-Azide Click Chemistry (SPAAC).

A number of (desthio) biotinylation reagents with

  • different solubility characteristics (PEG- and Sulfo-linker for increased molecule solubility)
  • different cleavability characteristics (chemically or photocleavable linke)
are available to fit specific application & molecule requirements.

*DBCO is also known as ADIBO (= Azadibenzocyclooctyne) or DIBAC (= Dibenzoazacyclooctyne)


Table 1: Available DBCO-containing (Desthio) Biotinylation Reagents

Biotin with chemically cleavable linkerDiazo Biotin-DBCO
Dde Biotin-DBCO
Biotin with photocleavable linkerPhotocleavable Biotin-DBCO