Adenosine-5'-[(β,γ)-imido]triphosphate, Tetralithium salt
For research use only!
Shipping: shipped on dry ice
Storage Conditions: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible.
Shelf Life: 6 months after date of delivery
Molecular Formula: C10H17N6O12P3 (free acid)
Molecular Weight: 506.19 g/mol (free acid)
Exact Mass: 506.01 g/mol (free acid)
Purity: ≥ 95 % (HPLC)
Color: white to off-white
Spectroscopic Properties: λmax 259 nm, ε 15.4 L mmol-1 cm-1 (Tris-HCl pH 7.5)
X-ray analysis[1, 2]
Hydrolyse studies[3, 4]Agonistic ligand, mainly for nucleoside receptor A1
Nucleosidephosphates stabilized against hydrolytic degradation can directly bind to nucleoside receptors.
Thymidylate kinase[1, 2]
for P2Y2 receptor
Please note: For reasons of stability, please make sure that the pH value of a solution of this product never drops below 7.0. This can be achieved by dissolving the nucleotide in a buffer of your choice (50 - 100 mM, pH 7 - 10). Dissolve and adjust concentration photometrically.
When stored at -20 °C, product may hydrolyze, thereby forming AppNH2 at a rate of up to 1 % per month!
Please click the black arrow on the right to expand the citation list. Click publication title for the full text.
 Segura-Pena et al. (2007) Quartenary structure change as a mechanism for the regulation of thymidine kinase 1-like enzymes. Structure 15:1555.
 Ostermann et al. (2003) Structure of human thhymidilate kinase in complex with prodrugs:Implications for the structure-based design of novel compounds. Biochemistry 42:2568.
 Shimizu et al. (1997) Hydrolysis of AMPPNP by the motor domain of NCD, a kinesin-related protein. Mol. Biol. Cell 8:1497.
 Suzuki et al. (1997) Hydrolysis of AMPPNP by the motor domain of ncd, a kinesin-related protein. FEBS Lett. 409 (1):29.
 Lazarowski et al. (1995) Pharmacological selectivity of cloned human P2U-purinoreceptor: potent activation by diadenosine tetraphosphate. Br. J. Pharmacol. 116 (1):1619.
Bodey et al. (2009) 9-Angström structure of a microtubule-bound mitotic motor. J. Mol. Biol. 388 (2):218.
Karow et al. (2009) A conformational change in the helicase core is necessary but not sufficient for RNA unwinding by the DEAD box helicase YxiN. Nucleic Acids Research 37 (13):4464.
Chang et al. (2005) Nitric Oxide-dependent Allosteric Inhibitory Role of a Second Nucleotide Binding Site in Soluble Guanylyl Cyclase. J. Biol. Chem. 280 (12):11513.
Menendez-Arias et al. (2005) Supression of Multidrug-resistant HIV-1 Reverse Transscriptase Primer Unblocking Activity by a-Phosphate-modified Thymidine Analogues. J. Mol. Biol. 349 (3):451.
Menz et al. (2001) The structure and nucleotide occupancy of bovine mitochondrial F-1-ATPase are not influenced by crystallisation at high concentrations of nucleotide. FEBS Lett. 494 (1):11.
Tereshko et al. (2001) Crystal structures of the catalytic domain of human protein kinase associated with apoptosis and tumor suppression. Nat. Struct. Biol. 8 (10):899.
Prodromou et al. (2000) The ATPase cycle of Hsp90 drives a molecular 'clamp' via transient dimerization of the N-terminal domains. EMBO J. 19 (16):4383.
Gil et al. (1999) N-Acetyl-L-glutamate kinase from Escherichia coli: cloning of the gene, purification and crystallization of the recombinant enzyme and preliminary X-ray analysis of the free and ligand-bound forms. Acta Cryst. D 55:1350.
Shi et al. (1999) EDC cross-linking of actin and myosin S1. II. AMPPNP induces an allosteric transition from pre-hydrolysis state to posthydrolysis state. Biophys. J. 76 (1):A163.
Williams et al. (1986) Effects of purine nucleotides on the binding of [3H]cyclopentyladenosine to adenosine A1-receptors in rat brain membranes. J. Neurochem. 47 (1):88.