For research use only!
Shipping: shipped at ambient temperature
Storage Conditions: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible.
Shelf Life: 12 months after date of delivery
Molecular Formula: C19H30N10O2
Molecular Weight: 430.51 g/mol
Exact Mass: 430.26 g/mol
Purity: > 95 % (HPLC)
Form: white to off-white solid
Solubility: water, DMSO, DMF, MeOH
BTTAA is a water-soluble, very effective ligand for Cu(I)-catalyzed Alkyne-Azide click chemistry reactions (CuAAC). It serves a dual purpose: 1) Accelaration of the CuAAC reaction by maintaining the Cu(I) oxidation state of copper sources and 2) Protection of biomolecules from oxidative damage during the labeling reaction[1,2].
BTTAA is a superior alternative to water-insoluble TBTA.
A stock solution can be prepared in ddH2O and subsequently be stored at -20°C. Avoid freeze-thaw cycles.
Presolski et al. and Hong et al. provide a general protocol for CuAAC reactions that may be used as a starting point for the set up and optimization of individual assays.
Please click the black arrow on the right to expand the citation list. Click publication title for the full text.
 Besanceney-Webler et al. (2011) Increasing the Efficiacy of Bioorthogonal Click Reactions for Bioconjugation: A Comparative Study Angew. Chem. Int. Ed. 50:8051.
 Uttamapinant et al. (2012) Fast, Cell-Compatible Click Chemistry with Copper-Chelating Azides for Biomolecular Labeling. Angew. Chem. Int. Ed. 51:5852.
 Presolski et al. (2011) Copper-Catalyzed Azide-Alkyne Click Chemistry for Bioconjugation. Current Protocols in Chemical Biology 3:153.
 Hong et al. (2011) Analysis and Optimization of Copper-Catalyzed Azide-Alkyne Cycloaddition for Bioconjugation. Angew. Chem. Int. Ed. 48:9879.