For general laboratory use.
Shipping: shipped at ambient temperature
Storage Conditions: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible.
Shelf Life: 12 months after date of delivery
Molecular Formula: C19H30N10O2
Molecular Weight: 430.51 g/mol
Exact Mass: 430.26 g/mol
Purity: ≥ 95 % (HPLC)
Color: white to off-white
Solubility: water, DMSO, DMF, MeOH
BTTAA is a water-soluble, very effective ligand for Cu(I)-catalyzed Alkyne-Azide click chemistry reactions (CuAAC). It serves a dual purpose: 1) Accelaration of the CuAAC reaction by maintaining the Cu(I) oxidation state of copper sources and 2) Protection of biomolecules from oxidative damage during the labeling reaction[1,2].
BTTAA is a superior alternative to water-insoluble TBTA.
A stock solution can be prepared in ddH2O and subsequently be stored at -20°C. Avoid freeze-thaw cycles.
Presolski et al. and Hong et al. provide a general protocol for CuAAC reactions that may be used as a starting point for the set up and optimization of individual assays.
BIOZ Product Citations:
 Besanceney-Webler et al. (2011) Increasing the Efficiacy of Bioorthogonal Click Reactions for Bioconjugation: A Comparative Study Angew. Chem. Int. Ed. 50:8051.
 Uttamapinant et al. (2012) Fast, Cell-Compatible Click Chemistry with Copper-Chelating Azides for Biomolecular Labeling. Angew. Chem. Int. Ed. 51:5852.
 Presolski et al. (2011) Copper-Catalyzed Azide-Alkyne Click Chemistry for Bioconjugation. Current Protocols in Chemical Biology 3:153.
 Hong et al. (2011) Analysis and Optimization of Copper-Catalyzed Azide-Alkyne Cycloaddition for Bioconjugation. Angew. Chem. Int. Ed. 48:9879.