5-Fluoro-uridine-5'-triphosphate, Sodium salt
For research use only!
Shipping: shipped on blue ice
Storage Conditions: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible.
Shelf Life: 12 months after date of delivery
Molecular Formula: C9H14N2O15P3F (free acid)
Molecular Weight: 502.13 g/mol (free acid)
Exact Mass: 501.96 g/mol (free acid)
Purity: ≥ 95 % (HPLC)
Form: clear aqueous solution
Concentration: 100 mM - 110 mM
pH: 7.5 ±0.5
Spectroscopic Properties: λmax 267 nm, ε 10.0 L mmol-1 cm-1 (Tris-HCl pH 7.5)
Inhibition of viral application
Conformational studies (NMR-NOE) of HIV-2 TAR-RNA
Use as NMR-label (F)
X-ray of viral RNA polymerase
 Agudo et al. (2008) Molecular Characterization of a Dual Inhibitory and Mutagenic Activity of 5-Fluorouridine Triphosphate on Viral RNA Synthesis. Implications for Lethal Mutagenesis. J. Mol. Biol. 382:652.
 Hennig et al. (2007) Synthesis of 5-fluoropyrimidine nucleotides as sensitive NMR probes of RNA structure. J. Am. Chem. Soc. 129:14911.
 Ferrer-Orta et al. (2007) Sequential structures provide insights into the fidelity of RNA replication. PNAS USA 104:9463.
Gilles Labesse et al. (2011) Structural and functional characterization of the Mycobacterium tuberculosis uridine monophosphate kinase: insights into the allosteric regulation. Nucleic Acids Res. 39 (8):3458.
Gilles et al. (2007) Regulatory Mechanisms Differ in UMP Kinases from Gram-negative and Gram-positive Bacteria. J. Biol. Chem. 282 (10):7242.
Au et al. (1982) Reversed-phase ion-pair high-performance liquid-chromatographic assay of 5-fluorouracil, 5'-deoxy-5- fluorouridine, their nucleosides, monophosphate, diphosphate, and triphosphate nucleotides with a mixture of quaternary ammonium-ions. J. Chromatogr. 228:245.
Glazer et al. (1980) The effect of 5-fluorouridine 5'-triphosphate on RNA transcribed in isolated-nuclei invitro. Mol. Pharmacol. 17 (2):279.
Anukarahanonta et al. (1979) Enhancement of 5-fluorouridine action in hepatoma-cells by galactosamine-induced uridine triphosphate deficiency. H-S Z. Physiol. Chem. 360 (3):225.