2-Ethynyl-ATP (2-EATP)

For general laboratory use.

Please centrifuge briefly before opening (volume ≤2 ml).

Description:
2-Ethynyl-labeled adenosine triphosphate (2-EATP) is suitable for in vitro polyadenylation of RNA with recombinant poly(A) polymerase^[1].

The resulting Alkyne-functionalized RNA can subsequently be processed via Cu(I)-catalyzed Azide-Alkyne click
chemistry (CUAAC) that offers the choice

• to introduce a Biotin group for subsequent purification tasks (via Azides
  of Biotin)
• to introduce fluorescent group for subsequent microscopic imaging (via
  Azides of fluorescent dyes)
• to crosslink the RNA to azide-functionalized biomolecules e.g.proteins

Presolski et al.^[2] and Hong et al.^[3] provide a general protocol for Cu(I)-catalyzed click chemistry reactions that may be used as a starting point for the set up and optimization of individual assays.

Shipping: shipped on gel packs

Storage Conditions: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible.

Shelf Life: 12 months after date of delivery

Molecular Formula: C₁₂H₁₆N₅O₁₃P₃

Molecular Weight: 531.20 g/mol

Exact Mass: 531.00 g/mol

Purity: ≥ 90 % (HPLC)

Form: solution in 100 mM Tris-HCl

Color: colorless to slightly yellow

Concentration: 10 mM - 11 mM

pH: 7.5 ±0.5

Spectroscopic Properties: λ_max 265 nm, ε 10.6 L mmol^-1 cm^-1 (Tris-HCl pH 7.5)

Applications:
in vitro polyadenylation of RNA^[1]

Related products:

• 5-Ethynyl-adenosine (5-EA), #CLK-N005
• Copper (II)-Sulphate (CuSO₄), #CLK-MI004
• Tris(3-hydroxypropyltriazolylmethyl)amine (THPTA), #CLK-1010
• Sodium Ascorbate (Na-Ascorbate), #CLK-MI005

Selected References:
[1] Curanovic et al. (2013) Global profiling of stimulus-induced polyadenylation in cells using a poly (A) trap. Nat. Chem. Biol. 9:671.
[2] Presolski et al. (2011) Copper-Catalyzed Azide-Alkyne Click Chemistry for Bioconjugation. Current Protocols in Chemical Biology 3:153.
[3] Hong et al. (2011) Analysis and Optimization of Copper-Catalyzed Azide-Alkyne Cycloaddition for Bioconjugation. Angew. Chem. Int. Ed. 48:9879.