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2-Ethynyl-adenosine

Cat. No. Amount Price (EUR) Buy / Note
CLK-N005-1 1 mg 75,54 Add to Basket/Quote Add to Notepad
CLK-N005-5 5 mg 292,19 Add to Basket/Quote Add to Notepad
Structural formula of 2-Ethynyl-adenosine
Structural formula of 2-Ethynyl-adenosine

For research use only!

Shipping: shipped at ambient temperature

Storage Conditions: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible.

Shelf Life: 12 months after date of delivery

Molecular Formula: C12H13N5O4

Molecular Weight: 291.26 g/mol

Purity: ≥ 95 % (HPLC)

Form: solid

Solubility: up to 4.0 mM in 1 x PBS

Applications:
mRNA poly(A) tail synthesis monitoring[1]

Description:
2-Ethynyl-labeled adenosine (2-EA) can be used to measure de novo mRNA poly(A) tail synthesis in proliferating cells. 2-EA is cell permeable and incorporates into nascent mRNA transcripts both transcriptionally by RNA polymerase I,II and III and posttranscriptionally by poly(A) polymerase instead of their natural analolg adenosine.

The resulting ethynyl-functionalized RNA can subsequently be detected via Cu(I)-catalyzed click chemistry that offers the choice to introduce a Biotin group (via Azides of Biotin) for subsequent purification tasks or a fluorescent group (via Azides of fluorescent dyes) for subsequent microscopic imaging[1].

Presolski et al.[2] and Hong et al.[3] provide a general protocol for Cu(I)-catalyzed click chemistry reactions that may be used as a starting point for the set up and optimization of individual assays.

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Selected References:
[1] Curanovic et al. (2013) Global profiling of stimulus-induced polyadenylation in cells using a poly (A) trap. Nat. Chem. Biol. 9 (11):671.
[2] Presolski et al. (2011) Copper-Catalyzed Azide-Alkyne Click Chemistry for Bioconjugation. Current Protocols in Chemical Biology 3:153.
[3] Hong et al. (2011) Analysis and Optimization of Copper-Catalyzed Azide-Alkyne Cycloaddition for Bioconjugation. Angew. Chem. Int. Ed. 48:9879.