» Sign in / Register

Enzymatic CLICK-functionalization of RNA


Nucleotide Selector for DNA/RNA Labeling
Select a suitable nucleotide substrate for enzymatic DNA/RNA Labeling.

Table 1: Enzymatic incorporation of CLICK-functionalized nucleotides.


cRNA Labeling
by in vitro Transcription with...
3'-RNA Labeling by...
NucleotideCat. No.T7 RNA Polymerase T3 RNA PolymeraseSP6 RNA PolymeraseyPAPTdTT4 RNA Ligase
UTP
5-Azidomethyl-UTPCLK-083n/an/an/an/an/an/a
5-Azido-C3-UTPNU-1571,2n/an/a---
5-Azido-PEG4-UTPCLK-054n/an/an/an/an/an/a
5-Ethynyl-UTPCLK-T081n/an/a---
DBCO-PEG4-UTPCLK-055n/an/an/an/an/an/a
Vinyl-UTPCLK-069n/an/an/an/an/an/a
ATP
8-Azido-ATPNU-155-- -2 - -
3'-Azido-2',3'-ddATPNU-882-- -2 - -
CTP
5-Azido-PEG4-CTPCLK-053n/an/an/an/an/an/a
5-DBCO-PEG4-CTPCLK-056n/an/an/an/an/an/a
3'-dATP (Cordycepin derivative)
N6-Azidohexyl-3'-dATPNU-1707-- -n/a - -
Cytidine-5'/3'-phosphate
pCp-AzideNU-1708-- -- - 2
CpG
5-DBCO-PEG4-dCpGCLK-062n/an/an/an/an/an/a
yPAP: Yeast Poly A Polymerase
TdT: Terminal deoxynucleotidyl Transferase.
1: in-house tested
2: external reference (see datasheet)
n/a: no data available
- : not applicable