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Cytidine-5'-phosphate-3'-(6-aminohexyl)phosphate, labeled with Cy3, Triethylammonium salt

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NU-1706-CY3 20 μl (1 mM) 150,10 Add to Basket/Quote Add to Notepad
Structural formula of pCp-Cy3 (Cytidine-5'-phosphate-3'-(6-aminohexyl)phosphate, labeled with Cy3, Triethylammonium salt)
Structural formula of pCp-Cy3
Excitation and Emission spectrum of Cy3
Excitation and Emission spectrum of Cy3

For general laboratory use.

Shipping: shipped on gel packs

Storage Conditions: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible.

Shelf Life: 12 months after date of delivery

Molecular Formula: C46H64N6O18P2S2 (free acid)

Molecular Weight: 1115.11 g/mol (free acid)

Exact Mass: 1114.32 g/mol (free acid)

Purity: ≥ 95 % (HPLC)

Form: solution in 10 mM Tris-HCl

Color: pink

Concentration: 1.0 mM - 1.1 mM

pH: 7.5 ±0.5

Spectroscopic Properties: λexc 550 nm, λem 570 nm, ε 150.0 L mmol-1 cm-1 (Tris-HCl pH 7.5)

Incorporation into RNA/miRNA by

  • 3'-End Labeling with T4 RNA Ligase[1]

pCp-Cy3 is recommended for 3'-End Labeling of RNA/miRNA with T4 RNA Ligase that conjugates a single dye molecule to the 3'-OH group of the RNA/miRNA template. The resulting Dye-labeled RNA/miRNA probes are ideally suited for hybridization experiments such as microarray-based expression profiling or electro mobility shift assays (EMSA).

Please note: Protect the Dye-labeled pCp from exposure to light and carry out experimental procedures under low light conditions.

Related products:

BIOZ Product Citations:

Selected References:
[1] Wanget al. (2007) Direct and sensitive miRNA profiling from low-input total RNA. RNA 13 (1):151.