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Fluorescent Nucleotides for RNA Labeling


Nucleotide Selector for DNA/RNA Labeling
Select a suitable nucleotide substrate for enzymatic DNA/RNA Labeling.

 

Table 1: Enyzmatic incorporation of fluorescent Nucleotides.



cRNA Labeling
by in vitro Transcription with...
3'-RNA Labeling by…
Emission
color
NucleotideCat. No.T7 RNA Polymerase T3 RNA PolymeraseSP6 RNA PolymeraseyPAPthTT4 RNA Ligase
UTP
blueUTP-PEG5-AF405NU-821-PEG5-AF4051n/an/an/an/a-
greenFluorescein-12-UTPNU-821-FAMX1n/an/an/an/a-
UTP-PEG5-AF488NU-821-PEG5-AF4881n/an/an/an/a-
UTP-ATTO-488NU-821-4881n/an/an/an/a-
yellow-
green
UTP-ATTO-532NU-821-5321n/an/an/an/a-
yellowUTP-Cy3NU-821-Cy3 2n/an/an/an/a-
UTP-X-Cy3NU-821-X-CY32n/an/an/an/a-
UTP-ATTO-550NU-821-5501n/an/an/an/a-
UTP-PEG5-AF555NU-821-PEG5-AF5551n/an/an/an/a-
orangeUTP-PEG5-AF594NU-821-PEG5-AF5941n/an/an/an/a-
redUTP-Cy5NU-821-Cy5 2n/an/an/an/a-
UTP-X-Cy5NU-821-X-CY5n/an/an/an/an/a-
UTP-PEG5-AF647NU-821-PEG5-AF6471n/an/an/an/a-
UTP-ATTO-647NNU-821-647N1n/an/an/an/a-
near-IRUTP-680NU-821-680 1,2n/an/an/an/a-
CTP
yellowCTP-Cy3NU-831-Cy32n/an/an/an/a-
redCTP-Cy5NU-831-Cy52n/an/an/an/a-
Cytidine-5’/3’-phosphate
greenpCp-AF488NU-1706-AF488-----2
pCp-488NU-1706-488-----2
yellowpCp-Cy3NU-1706-Cy3-----2
pCp-AF555NU-1706-AF555-----2
orangepCp-AF594NU-1706-AF594-----2
redpCp-Cy5NU-1706-Cy5-----2
pCp-643NU-1706-643-----2
pCp-AF647NU-1706-AF647-----2

yPAP: Yeast Poly A Polymerase
TdT: Terminal deoxynucleotidyl Transferase.
1: in-house tested
2: external reference (see datasheet)
n/a: no data available
- : not applicable