T4 dNMP (deoxy-Nucleotide Monophosphate) Kinase

For general laboratory use.

Please centrifuge briefly before opening (volume ≤2 ml).

Description:
NMP kinases catalyse the phosphorylation of nucleotide monophosphates resulting in the corresponding nucleotide diphosphates by using ATP as a phosphate donor. T4 dNMP kinase is the only member of the family of NMP kinases that recognizes three structurally dissimilar nucleotides: dGMP, dTMP and 5-hydroxymethyl-dCMP (5-OH-dCMP).

Shipping: shipped on gel packs

Storage Conditions: store at -20 °C
avoid freeze/thaw cycles

Shelf Life: 12 months

Molecular Weight: 27 kDa

Accession number: AAD42414

Purity: > 90 % (SDS-PAGE)

Form: liquid (Supplied in 100 mM Tris-HCl pH 7.5, 10 mM MgCl₂ and 50 % glycerol)

Activity: One unit of enzyme catalyses the phosphorylation of 1 nmol dGMP to dGDP per minute at 37°C. Specific activity: 100 u/μg.

Activity Assay: 1 μg T4 dNMP kinase, 2 mM ATP and 2 mM dGMP in 100 mM Tris-HCl pH 7.5, 10 mM MgCl₂.

Selected References:
Teplyakov et al. (1996) Crystal structure of bacteriophage T4 deoxynucleotide kinase with its substrates dGMP and ATP. EMBO J. 15:3487.
Brush et al. (1993) Chemical modification of bacteriophage T4 deoxynucleotide kinase. Evidence of a single catalytic region. J. Biol. Chem. 268:1603.
Brush et al. (1990) Bacteriophage T4 deoxynucleotide kinase: gene cloning and enzyme purification. J. Bacteriol. 172:2935.