Cytidine-5'-phosphate-3'-(6-aminohexyl)phosphate, labeled with Cy5, Triethylammonium salt
For research use only!
Shipping: shipped on blue ice
Storage Conditions: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible.
Shelf Life: 12 months after date of delivery
Molecular Formula: C48H66N6O18P2S2 (free acid)
Molecular Weight: 1141.14 g/mol (free acid)
Exact Mass: 1140.34 g/mol (free acid)
Purity: ≥ 95 % (HPLC)
Form: blue solution in 10 mM Tris-HCl
Concentration: 1.0 mM - 1.1 mM
pH: 7.5 ±0.5
Spectroscopic Properties: λexc 649 nm, λem 670 nm, ε 250.0 L mmol-1 cm-1 (Tris-HCl pH 7.5)
Incorporation into RNA/miRNA by
pCp-Cy5 is recommended for 3'-End Labeling of RNA/miRNA with T4 RNA Ligase that conjugates a single dye molecule to the 3'-OH group of the RNA/miRNA template. The resulting Dye-labeled RNA/miRNA probes are ideally suited for hybridization experiments such as microarray-based expression profiling or electro mobility shift assays (EMSA).
Please note: Protect the Dye-labeled pCp from exposure to light and carry out experimental procedures under low light conditions.
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 Wang et al. (2007) Direct and sensitive miRNA profiling from low-input total RNA. RNA 13 (1):151.